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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Characterization and profiling analysis of bovine oviduct and uterine extracellular vesicles and their miRNA cargo through the estrous cycle

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Hamdi, Meriem [1, 2] ; Canon-Beltran, Karina [1, 3] ; Mazzarella, Rosane [4] ; Cajas, Yulia N. [1, 5] ; Leal, Claudia L. V. [4] ; Gutierrez-Adan, Alfonso [1] ; Gonzalez, Encina M. [6] ; Da Silveira, Juliano C. [4] ; Rizos, Dimitrios [1]
Total Authors: 9
[1] INIA CSIC, Dept Anim Reprod, Madrid - Spain
[2] Univ Zurich, Vetsuisse Fac, Dept Farm Anim, Genet & Funct Genom, Clin Reprod Med, Zurich - Switzerland
[3] Fdn Univ Juan Castellanos, Programa Med Vet, Fac Ciencias Agr & Ambientales, Tunja - Colombia
[4] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, Pirassununga - Brazil
[5] Univ Cuenca UC, Fac Ciencias Agr, Lab Biotecnol Reprod Anim, Cuenca - Ecuador
[6] Complutense Univ Madrid UCM, Fac Vet, Dept Anat & Embryol, Madrid - Spain
Total Affiliations: 6
Document type: Journal article
Source: FASEB JOURNAL; v. 35, n. 12 DEC 2021.
Web of Science Citations: 0

Extracellular vesicles (EVs) found in various biological fluids and particularly in reproductive fluids, have gained considerable attention for their possible role in cell- to- cell communication. Among, the different bioactive molecules cargos of EVs, MicroRNAs (miRNAs) are emerging as promising diagnostic biomarkers with high clinical potential. Aiming to understand the roles of EVs in bovine reproductive tract, we intended to characterize and profile the EVs of oviduct and uterine fluids (OF-EVs, UF-EVs) and their miRNA across the estrous cycle. Nanoparticle tracking analysis and transmission electron microscopy confirmed the existence of small EV population in OF and UF at all stages, (size between 30 and 200 nm; concentration: 3.4 x 10(10) EVs/ml and 6.0 x 10(10) EVs/ml for OF and UF, respectively, regardless of stage). The identification of EV markers (CD9, HSP70, and ALIX proteins) was confirmed by western blot. The miRNA analysis revealed the abundance of 310 and 351 miRNAs in OF-EVs and UF-EVs, respectively. Nine miRNAs were differentially abundant in OF-EVs between stages of the cycle, eight of them displayed a progressive increase from S1 to S4 (p < .05). In UF-EVs, a total of 14 miRNAs were differentially abundant between stages. Greater differences were observed between stage 1 (S1) and stage 3 (S3), with 11 miRNAs enriched in S3 compared to S1. Functional enrichment analysis revealed the involvement of these miRNAs in relevant pathways such as cell signaling, intercellular junctions, and reproductive functions that may be implicated in oviduct and uterus modulation across the cycle, but also in their preparation for embryo/conceptus presence and development. (AU)

FAPESP's process: 17/20339-3 - Influence of extracellular vesicles on lipid metabolism, development and quality of bovine embryos produced in vitro
Grantee:Cláudia Lima Verde Leal
Support type: Scholarships abroad - Research
FAPESP's process: 19/04981-2 - Effects of extracellular vesicle supplementation during bovine embryo cleavage
Grantee:Rosane Mazzarella
Support type: Scholarships in Brazil - Master
FAPESP's process: 15/21829-9 - Multi-user equipment approved in grant 2014/22887-0: Nanosight LM10
Grantee:Juliano Coelho da Silveira
Support type: Multi-user Equipment Program
FAPESP's process: 14/22887-0 - Cell-secreted vesicles containing miRNAs modulate epigenetic changes during in vitro culture of bovine gametes and embryos
Grantee:Juliano Coelho da Silveira
Support type: Research Grants - Young Investigators Grants