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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The peptide secreted at the water to land transition in a model amphibian has antioxidant effects

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Author(s):
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Barbosa, Eder Alves [1, 2] ; Placido, Alexandra [3, 4] ; Moreira, Daniel C. [5] ; Albuquerque, Lucas [6] ; Dematei, Anderson [5, 7] ; Silva-Carvalho, Amandda E. [8] ; Cabral, Wanessa F. [5] ; Bao, Sonia N. [9] ; Saldanha-Araujo, Felipe [8] ; Kuckelhaus, Selma A. S. [5] ; Borges, Tatiana K. [6] ; Portugal, Camila C. [10, 11] ; Socodato, Renato [10, 11] ; Teixeira, Catia [3] ; Lima, Filipe Camargo D. A. [12] ; Batagin-Neto, Augusto [13] ; Sebben, Antonio [14] ; Eaton, Peter [3, 15] ; Gomes, Paula [3] ; Brand, Guilherme D. [2] ; Relvas, Joao B. [10, 11] ; Kato, Massuo J. [16] ; Leite, Jose Roberto S. A. [5, 4]
Total Authors: 23
Affiliation:
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[1] EMBRAPA Recursos Genet & Biotecnol, Lab Espectrometria Massa, Brasilia, DF - Brazil
[2] Univ Brasilia, Inst Quim, Lab Sintese & Anal Biomol, Brasilia, DF - Brazil
[3] Univ Porto, Fac Ciencias, Dept Quim & Bioquim, LAQV REQUIMTE, Porto - Portugal
[4] Bioprospectum Lda, UPTEC, Porto - Portugal
[5] Univ Brasilia, Nucleo Pesquisa Morfol & Imunol Aplicada NuPMIA, Fac Med, Brasilia, DF - Brazil
[6] Univ Brasilia, Lab Imunol Celular, Fac Med, NuPMIA, Brasilia, DF - Brazil
[7] Univ Brasilia, Fac Med, Nucleo Med Trop, Programa Posgrad Med Trop, Brasilia, DF - Brazil
[8] Univ Brasilia, Fac Ciencias Saude, Lab Hematol & Celulas Tronco, Brasilia, DF - Brazil
[9] Univ Brasilia, Lab Microscopia & Microanal, Inst Ciencias Biol, Brasilia, DF - Brazil
[10] Univ Porto, Inst Biol Mol & Celular, Porto - Portugal
[11] Univ Porto, Glial Cell Biol Lab, Inst Invest & Inovacao Saude, Porto - Portugal
[12] Inst Fed Educ Ciencia & Tecnol Sao Paulo, Matao - Brazil
[13] Sao Paulo State Univ UNESP, Campus Itapeva, Itapeva - Brazil
[14] Univ Brasilia, Inst Ciencias Biol, Brasilia, DF - Brazil
[15] Univ Lincoln, Joseph Banks Labs, Sch Chem, The Bridge, Lincoln - England
[16] Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, Sao Paulo, SP - Brazil
Total Affiliations: 16
Document type: Journal article
Source: PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES; v. 288, n. 1962 NOV 10 2021.
Web of Science Citations: 0
Abstract

In addition to the morphophysiological changes experienced by amphibians during metamorphosis, they must also deal with a different set of environmental constraints when they shift from the water to the land. We found that Pithecopus azureus secretes a single peptide ({[}M + H]+ = 658.38 Da) at the developmental stage that precedes the onset of terrestrial behaviour. De novo peptide and cDNA sequencing revealed that the peptide, named PaT-2, is expressed in tandem and is a member of the tryptophyllins family. In silico studies allowed us to identify the position of reactive sites and infer possible antioxidant mechanisms of the compounds. Cell-based assays confirmed the predicted antioxidant activity in mammalian microglia and neuroblast cells. The potential neuroprotective effect of PaT-2 was further corroborated in FRET-based live cell imaging assays, where the peptide prevented lipopolysaccharide-induced ROS production and glutamate release in human microglia. In summary, PaT-2 is the first peptide expressed during the ontogeny of P. azureus, right before the metamorphosing froglet leaves the aquatic environment to occupy terrestrial habitats. The antioxidant activity of PaT-2, predicted by in silico analyses and confirmed by cell-based assays, might be relevant for the protection of the skin of P. azureus adults against increased O-2 levels and UV exposure on land compared with aquatic environments. (AU)

FAPESP's process: 18/07999-7 - VIDA-FROG: valorization of molecules isolated from aquatic Portuguese amphibians
Grantee:Massuo Jorge Kato
Support type: Regular Research Grants