Goncalves, Alexandre S. R.
Campos, Ana Carolina P.
Assis, V, Daniele
Kuroki, Mayra A.
Sant'Anna, Fernando M.
Pagano, Rosana L.
Total Authors: 11
 Hosp Sirio Libanes, Lab Neurosci, Rua Prof Daher Cutait 69, BR-01308060 Sao Paulo, SP - Brazil
 Univ Sao Paulo, Hosp Clin, Acupuncture Ctr, Med Sch, Sao Paulo - Brazil
 Assis, Daniele, V, Hosp Sirio Libanes, Lab Neurosci, Rua Prof Daher Cutait 69, BR-01308060 Sao Paulo, SP - Brazil
 Univ Fed Rio de Janeiro, Macae - Brazil
 Ctr Evaluat & Traitement Douleur CETD, Nantes - France
 Ctr Hosp Univ Nantes, Nantes - France
 Int Coll Auriculomed & Auriculotherapy Review, Cd Lazaro Cardenas, Michoacan - Mexico
Total Affiliations: 7
Acupuncture in Medicine;
Web of Science Citations:
Background: Neuropathic pain (NP) is a complex disease that remains challenging to treat. Low-frequency dense-and-disperse (DD) electroacupuncture (EA) has been used as adjuvant therapy for neuropathic pain; however, its analgesic effect decreases as stimulation time increases, or when it is repeatedly used. We hypothesized that a new frequency parameter could improve the effectiveness of EA, and aimed to compare the efficacy and duration of the analgesic effect between classic DD-EA and non-repetitive and non-sequential frequency (random frequency (RF)-EA) in neuropathic rats. Furthermore, the effect of RF-EA at local traditional acupuncture point locations versus auricular vagus nerve stimulation (aVNS) was evaluated. Methods: Male Wistar rats with peripheral neuropathy were subjected to a single session of DD-EA or RF-EA for 20 or 40 min at ST36 + GB34. An additional group of rats was treated with RF-EA for 20 min using aVNS at the appropriate ear point locations. Paw pressure test, von Frey filaments and spontaneous pain scores were evaluated. Sham-operated rats were used as controls. Results: In all, 20 min of RF-EA reversed hyperalgesia (for 24 h) and allodynia (for 8 h), showing a longer analgesic effect than DD-EA. Both RF-EA and DD-EA induced partial inhibition of spontaneous pain for 8 h. Forty minutes of DD-EA did not interfere with the NP phenomena; however, RF-EA induced significant long-term analgesia. aVNS induced an analgesic effect similar to local stimulation. Conclusion: This pilot study shows that RF-EA at both local traditional acupuncture point and auriculotherapy point locations induces long-lasting analgesia in neuropathic rats, and more effectively so than classical DD-EA. (AU)