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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

he use of probiotics can reduce the severity of experimental periodontitis in rats with metabolic syndrome: An immunoenzymatic and microtomographic stud

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Silva, Giselle A. [1] ; Moreira, Andre L. G. [1] ; Silva, Pedro H. F. [1] ; Salvador, Sergio L. [2] ; Casarin, V, Renato C. ; Vicente, Raphael M. [3] ; Ferreira, Graziele C. [4] ; Tanus-Santos, Jose E. [4] ; Furlaneto, Flavia A. C. [1] ; Messora, Michel R. [1]
Total Authors: 10
[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Oral & Maxillofacial Surg & Periodontol, USP, Av Cafe S-N, BR-14020150 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Anal, USP, Ribeirao Preto, SP - Brazil
[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Orthoped & Anesthesiol, USP, Ribeirao Preto, SP - Brazil
[4] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pharmacol, USP, Ribeirao Preto, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Journal of Periodontology; v. 93, n. 2 NOV 2021.
Web of Science Citations: 0

Background This study evaluated the effects of Bifidobacterium animalis subsp. lactis HN019 (B. lactis HN019) in the development of periodontitis (PE), associated or not with metabolic syndrome, (MS) in rats. Methods Ninety-six rats were grouped according to a food protocol: high-fat diet for induction of MS or standard diet for the control groups (C). They were subdivided into groups with (+) and without (-) PE, receiving ({*}) or not ({*}{*}) probiotic (PROB): C-{*}{*}, CP-{*}, PE+{*}{*}, PEP+{*}, MS- MSP-{*}, MSPE+{*}{*}, and MSPEP+{*}. PROB administration started on the eighth week of the study and PE was induced on the 14th week by placing ligature on the animals' lower first molars. Euthanasia occurred in the 16th week. Biomolecular analyzes, immunoenzymatic assays, and microtomographic analyses were performed. The data obtained were analyzed statistically (P < 0.05). Results The PEP and MSPEP groups showed lower levels of alveolar bone loss when compared with the PE and MSPE groups, respectively (P < 0.05). The immunoenzymatic analysis showed higher levels of interleukin (IL)-1 beta and a higher receptor activator of NF-kappaB ligand (RANKL)/osteoprotegerin (OPG) ratio in the MSPE group when compared with the MSPEP group (P < 0.05). The PEP group showed lower levels of tumor necrosis factor (TNF)-alpha and IL-6 when compared with the PE group. The use of PROB attenuated dyslipidemia parameters in animals with MS, with or without PE. Conclusion B. lactis HN019 reduced more significantly the severity of PE in rats with MS, modulating both systemic metabolic and immunoinflammatory parameters in periodontal tissues. (AU)

FAPESP's process: 18/16009-0 - Effects of probiotic therapy on experimental periodontitis in rats fed with a high-fat diet
Grantee:Giselle Aparecida da Silva
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 17/26257-9 - Effects of the therapy with Bifidobacterium animalis subsp. lactis HN019 on experimental periodontitis associated with Metabolic Syndrome. Microtomographic, histomorphometric, immunoenzymatic and gene expression profile study in rats.
Grantee:Michel Reis Messora
Support Opportunities: Regular Research Grants