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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

NS1-based ELISA test efficiently detects dengue infections without cross-reactivity with Zika virus

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Pereira, Samuel Santos [1] ; Andreata-Santos, Robert [1] ; Pereira, Lennon Ramos [1] ; Soares, Camila Pereira [2] ; Felix, Alvina Clara [3] ; Jungmann Cardoso de Andrade, Patricia de Mello [4] ; Durigon, Edison Luis [2] ; Romano, Camila Malta [3, 5] ; de Souza Ferreira, Luis Carlos [1]
Total Authors: 9
[1] Univ Sao Paulo, Inst Biomed Sci, Microbiol Dept, Vaccine Dev Lab, Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Biomed Sci, Microbiol Dept, Lab Clin & Mol Virol, Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Med Trop Sao Paulo, Sao Paulo - Brazil
[4] Univ Pernambuco UPE, Gen Pathol, Recife, PE - Brazil
[5] Univ Sao Paulo, Hosp Clin HCFMUSP LIM 52, Fac Med, Sao Paulo - Brazil
Total Affiliations: 5
Document type: Journal article
Web of Science Citations: 0

Objectives: The aim of this study was to achieve greater specificity of dengue virus (DENV) serological tests based on a recombinant antigen derived from non-structural protein 1 (Delta NS1) with regard to cross-reactive Zika virus (ZIKV) anti-NS1 antibody responses. This is of relevance in endemic regions for the serological discrimination of both DENV and ZIKV, such as Brazil and other tropical countries. Methods: The Delta NS1 proteins were obtained as recombinant antigens and were evaluated as solid-phase-bound antigens in the ELISA test to detect anti-NS1 IgG antibodies. The performance of the Delta NS1-based DENV IgG ELISA was assessed with both mouse and human serum samples previously exposed to DENV or ZIKV. Results: The Delta NS1-based DENV IgG ELISA detected anti-DENV NS1 IgG without cross-reactivity with ZIKV-positive serum samples. The sensitivity and specificity of the assay determined using samples previously characterized by real-time PCR (qRT-PCR) or plaque reduction neutralization assay (PRNT) were 82% and 93%, respectively. Conclusion: The Delta NS1-based DENV IgG ELISA conferred enhanced diagnostic specificity for anti-DENV serological tests and may be particularly useful for serological analyses in endemic regions for both DENV and ZIKV transmission. (C) 2021 The Authors. Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. (AU)

FAPESP's process: 17/09661-0 - Development of new serological diagnostic strategies for typing prior Dengue virus infection
Grantee:Samuel Santos Pereira
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 16/05570-8 - Use of Zika virus recombinant proteins for development of diagnostic methods and vaccines
Grantee:Lennon Ramos Pereira
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 16/20045-7 - Antigen discovery and development of serological diagnostic methods and vaccine approaches against the Zika Virus (ZIKV)
Grantee:Luis Carlos de Souza Ferreira
Support Opportunities: Research Projects - Thematic Grants