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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In vitro and in silico cytotoxicity of hinokinin-loaded PLGA microparticle systems against tumoral SiHa cells

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Author(s):
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de Lima, Regiane G. [1] ; Lisoni, Flavia C. R. [2] ; Picao, Thais B. [2] ; dos Santos, Fransergio F. [3] ; Orenha, Renato P. [3] ; Borges, Alexandre [4] ; Molina, Eduardo F. [3] ; Parreira, Renato L. T. [3] ; e Silva, Marcio L. A. [3] ; Santos, Mario F. C. [3] ; de Laurentiz, Rosangela da S. [1]
Total Authors: 11
Affiliation:
[1] Univ Estadual Paulista, Fac Engn Ilha Solteira, Dept Fis & Quim, Ilha Solteira, SP - Brazil
[2] Univ Estadual Paulista, Fac Engn Ilha Solteira, Dept Biol & Zootecnia, Ilha Solteira, SP - Brazil
[3] Univ Franca, Nucleo Pesquisa Ciencias Exatas & Tecnol, Franca, SP - Brazil
[4] Ctr Univ, Santa Fe Do Sul, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: NATURAL PRODUCT RESEARCH; NOV 2021.
Web of Science Citations: 0
Abstract

This work aimed to synthesize poly (D, L-lactic-co-glycolic acid) (PLGA) microparticles containing hinokinin (HNK) and to evaluate their cytotoxic activity against tumoral SiHa cells and non-tumoral HaCaT cells. Hinokinin was incorporated into PLGA (PLGA-HNK) with an encapsulation efficiency of 84.18 +/- 2.32%. PLGA and PLGA-HNK were characterized by SEM microscopy and showed spherical morphology with an average size of similar to 3.33. Encapsulation efficiency was determined by a calibration curve using UV-vis spectroscopy. PLGA-HNK more active inhibiting proliferation of SiHa cells (IC50 = 14.68 mu M) than free HNK (IC50 = 225.5 mu M). In relation to HaCaT cells, PLGA-HNK showed no significant difference compared to the negative control. These results led to an increase in HNK bioavailability and thereby, biological activity. In silico prediction analysis suggests that HNK is cytotoxic against SiHa cells with E6 and MDM2 inhibition as possible main mechanism of action. (AU)

FAPESP's process: 14/07493-5 - Synthesis and biological evaluation of heterocyclic compounds, analogs of lignans, obtained by microwave-assisted multi-component reactions
Grantee:Rosangela da Silva de Laurentiz
Support Opportunities: Regular Research Grants
FAPESP's process: 18/25010-2 - Isolation and identification of green and brown propolis constituents.
Grantee:Mário Ferreira Conceição Santos
Support Opportunities: Scholarships in Brazil - Post-Doctoral