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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Impacts of high-intensity exercise on the metabolomics profile of human skeletal muscle tissue

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Author(s):
Zagatto, Alessandro M. [1] ; Bishop, David J. [2] ; Antunes, Barbara Moura [1] ; Beck, Wladimir R. [3] ; Malta, Elvis S. [1] ; de Poli, Rodrigo A. B. [1] ; Cavaglieri, Claudia R. [4] ; Chacon-Mikahil, Mara Patricia T. [4] ; Castro, Alex [4]
Total Authors: 9
Affiliation:
[1] Sao Paulo State Univ UNESP, Lab Physiol & Sport Performance LAFIDE, Bauru, SP - Brazil
[2] Victoria Univ, Inst Hlth & Sport iHeS, Footscray, Vic - Australia
[3] Fed Univ Sao Carlos UFSCar, Sao Carlos - Brazil
[4] Univ Campinas UNICAMP, Fac Phys Educ, Exercise Physiol Lab, Campinas - Brazil
Total Affiliations: 4
Document type: Journal article
Source: SCANDINAVIAN JOURNAL OF MEDICINE & SCIENCE IN SPORTS; v. 32, n. 2 NOV 2021.
Web of Science Citations: 0
Abstract

The study aimed to identify and quantify the metabolites profile and metabolic pathways in human muscle tissue engaged during exhaustive high-intensity cycling exercise. Seven healthy physically active men performed a graded exercise test and an exhaustive supramaximal effort at 115% of maximal aerobic power with muscles biopsies performed in rest and immediately after exhaustion for quantifying of muscle metabolites changes by H-1-NMR spectroscopy. The time until exhaustion (tlim) recorded was 224.7 +/- 35.5 s whereas the muscle pH at exhaustion was 6.48 +/- 0.05. A total of 54 metabolites were identified and quantified. The most enriched and impacted pathways included: beta oxidation of very long chain fatty acids, mitochondrial electron transport chain, alanine aspartate, and glutamate metabolism, citric acid cycle, arginine biosynthesis, propanoate metabolism, threonine and 2-oxobutanoate degradation and pyruvate metabolism. In addition, the muscle concentrations in Post exercise, compared to Pre increased significantly (p < 0.0398) for fumarate (42.0%), succinate (101.2%), glucose (249.7%), lactate (122.8%), O-acetylcarnitine (164.7%), glycerol (79.3%), AMP (288.2%), 2-oxobutyrate (121.0%), and methanol (58.5%), whereas decreased significantly (p < 0.010) for creatine phosphate (-70.2%), ADP (-56.5%), carnitine (-33.5%), and glutamate (-42.3%). Only the succinate was significantly correlated with tlim (r = -0.76; p = 0.0497). Besides the classical expected contribution of glycolytic and phosphagen energetic pathways, it was demonstrated that the high-intensity exercise is also associated with pathways indicatives of amino acid and fatty acid oxidation metabolisms, highlighting the inverse relation between changes in the intramuscular succinate levels and tlim. (AU)

FAPESP's process: 20/13939-7 - Metabolomic analysis by Nuclear Magnetic Resonance (NMR) and mass spectrometry (LC-MS) of blood samples from individuals submitted to cardiorespiratory rehabilitation.
Grantee:Alex Castro
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 17/21724-8 - Impact of cold-water immersion regular use after high-intensity interval training sessions on muscle repair, muscle adaptation and running performance
Grantee:Elvis de Souza Malta
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 19/25282-5 - B-alanine supplementation associated with high-intensity interval training on modulations of the immune-endocrine response in healthy adults
Grantee:Barbara de Moura Mello Antunes
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 18/21126-6 - Fatigue process during supramaximal effort: Analysis of central and peripherical mechanisms, muscular acidosis, energetic substrate, enzymatic activity and metabolic
Grantee:Alessandro Moura Zagatto
Support type: Regular Research Grants