Oliveira, Luiz M.
Takakura, Ana C.
Moreira, Thiago S.
Total Authors: 3
 Univ Sao Paulo, Inst Ciencias Biomed, Dept Pharmacol, BR-05508000 Sao Paulo, SP - Brazil
 Seattle Childrens Res Inst, Ctr Integrat Brain Res, 1900 9th Ave, JMB10, Seattle, WA 98101 - USA
 Univ Sao Paulo, Inst Ciencias Biomed, Dept Physiol & Biophys, 1524 Lineu Prestes Ave, BR-05508000 Sao Paulo, SP - Brazil
Total Affiliations: 3
NOV 10 2021.
Web of Science Citations:
postinspiratory complex (PiCo) is a region located in the ventromedial medulla involved with the post-inspiratory activity. PiCo neurons are excitatory (VGlut2+) and express the enzyme choline acetyl transferase (ChAT+). Evidence also suggests that PiCo is coupled to two additional groups of neurons involved in breathing process, i.e. the pre-Botzinger complex (preBotC, inspiration) and the retrotrapezoid nucleus (RTN, active expiration), composing all together, the hypothesized triple respiratory oscillator. Here, our main objective is to demonstrate the afferent connections to PiCo region. We mapped projecting-neurons to PiCo by injecting Fluorogold (FG) retrograde tracer into the PiCo of adult Long-Evans Chat-cre male rats. We reported extensive projections from periaqueductal grey matter and Kolliker-Fuse regions and mild projections from the nucleus of the solitary tract, ventrolateral medulla and hypothalamus. We also injected a cre-dependent vector expressing channelrhodopsin 2 (AAV5-ChR2) fused with enhanced mCherry into the PiCo of ChAT-cre rats to optogenetic activate those neurons and investigate the role of PiCo for inspiratory/postinspiratory activity. Both in urethaneanesthetized and unrestrained conscious rats the response of ChR2-transduced neurons to light induced an increase in postinspiratory activity. Our data confirmed that PiCo seems to be dedicated to postinspiratory activity and represent a site of integration for autonomic and motor components of respiratory and non-respiratory pathways. (c) 2021 IBRO. Published by Elsevier Ltd. All rights reserved. (AU)