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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Simultaneous saccharification isomerization and Co-fermentation - SSICF: A new process concept for second-generation ethanol biorefineries combining immobilized recombinant enzymes and non-GMO Saccharomyces

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Xavier Guilherme, Ederson Paulo [1] ; Zanphorlin, Leticia Maria [2] ; Sousa, Amanda Silva [2] ; Miyamoto, Renan Yuji [3, 2] ; Oliveira Bruziquesi, Carlos Giovani [4] ; Aparecida de Carvalho Mesquita, Bruna Mara [1] ; Sousa Santos, Sergio Henrique [1] ; Aguiar-Oliveira, Elizama [5] ; Cota, Junio [1]
Total Authors: 9
[1] Univ Fed Minas Gerais, Inst Agr Sci, Montes Claros, MG - Brazil
[2] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Biorenewables Natl Lab LNBR, Campinas, SP - Brazil
[3] Univ Estadual Campinas, Fac Pharmaceut Sci, Campinas, SP - Brazil
[4] Univ Fed Minas Gerais, Dept Chem, Belo Horizonte, MG - Brazil
[5] Univ Estadual Santa Cruz, Dept Exact Sci & Technol, Ilheus, BA - Brazil
Total Affiliations: 5
Document type: Journal article
Source: RENEWABLE ENERGY; v. 182, p. 274-284, JAN 2022.
Web of Science Citations: 0

Integrated bioprocess strategies may facilitate ethanol production from both C6 and C5 fractions of lignocellulosic feedstocks. We propose a new process concept, SSICF, where sugarcane bagasse is hy-drolyzed simultaneously with xylose isomerization and the co-fermentation of C6 and C5 sugars. A commercial cocktail was supplemented with our multi-enzymatic system composed of three recombi-nant enzymes immobilized in Feroxyhyte magnetic nanoparticles: b-glucosidase, b-xylosidase and xylose isomerase. SSICF was performed using non-GMO Saccharomyces at pH 6.0 and 35 degrees C for 72 h in a synthetic medium containing cellobiose and xylose, and another medium containing pretreated sugar-cane bagasse (PSB). The results of ethanol global yields in SSICF were 77.67% and 73.24% for the synthetic medium and PSB, respectively. In a nutshell, this is the first report of a successful proof-of-concept of SSICF with four rounds of enzyme recycling and a non-GMO yeast, an innovative process with high potential for industrial use. (C) 2021 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 18/02865-2 - Molecular investigation of new xylose isomerases for application to lignocellulosic materials fermentation
Grantee:Renan Yuji Miyamoto
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 19/08855-1 - New mechanisms of P450: an enzymatic strategy for renewable hydrocarbons
Grantee:Leticia Maria Zanphorlin
Support type: Regular Research Grants