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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Whole-genome sequencing reveals high genetic diversity of Streptococcus uberis isolated from cows with mastitis

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Silva, Nathalia Cristina Cirone [1, 2] ; Yang, Yongqiang [3, 4] ; Rodrigues, Marjory Xavier [1] ; Tomazi, Tiago [1] ; Bicalho, Rodrigo Carvalho [1]
Total Authors: 5
[1] Cornell Univ, Coll Vet Med, Dept Populat Med & Diagnost Sci, Ithaca, NY 14853 - USA
[2] Univ Campinas UNICAMP, Fac Food Engn FEA, Dept Food Sci & Nutr, Rua Monteiro Lobato 80, BR-13083862 Campinas, SP - Brazil
[3] Sun Yat Sen Univ, Zhongshan Sch Med, Dept Microbiol, Guangzhou 510080 - Peoples R China
[4] Sun Yat Sen Univ, Sch Pharmaceut Sci Shenzhen, Guangzhou 510006 - Peoples R China
Total Affiliations: 4
Document type: Journal article
Source: BMC Veterinary Research; v. 17, n. 1 OCT 7 2021.
Web of Science Citations: 0

Background Bovine mastitis is an important cause of economic loss in dairy farms. Streptococcus uberis is among the most frequently isolated bacterial species isolated from cows with mastitis. The aim of this study was to perform an in-depth genetic assessment of S. uberis strains isolated from bovine clinical mastitis (CM) and to perform a phylogenetic analysis to represent the evolutionary relationship among S. uberis sequences. Results A total of 159 isolates was genetically characterized using whole genome sequencing. According to the virulence determinants, all strains harbored the hasC, leuS, perR, purH, and purN virulence genes. Thirty-four resistance genes were identified in at least one strain. In terms of acquired genes, we observed that 152 (95.6 %) strains had a resistance gene to lincosamine (lnuD), 48 (30.2 %) to tetracycline (tetM), 4 (2.51 %) to tobramicine (ant6), and 1 to lincosamide (lsa(E)). MLST detected the Sequence Type (ST)797 (n = 23), while 85.5 % of the strains did not match to known STs. Conclusions Then, eleven distinct ST were identified after we submitted the new alleles to assign new STs. The other prevalent STs observed were ST1215 (n = 58), ST1219 (n = 35), and ST1213 (n = 15). And it was not possible to identify the MLST of four strains. Phylogenetic lineages indicated a high genomic diversity of S. uberis in our collection, confirming that most strains isolated from bovine mastitis have different reservoirs, typical of environmental pathogens. (AU)

FAPESP's process: 18/24191-3 - Genomic investigation of coagulase-negative staphylococci isolated from mastitis cows
Grantee:Nathalia Cristina Cirone Silva
Support Opportunities: Scholarships abroad - Research