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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Comparative metavirome analysis in polytransfused patients

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Author(s):
I.N. Valença ; F.A. Rós ; V.S. Zucherato [3] ; A.C. Silva-Pinto [4] ; D.T. Covas [5] ; S. Kashima [6] ; S.N. Slavov
Total Authors: 7
Document type: Journal article
Source: Brazilian Journal of Medical and Biological Research; v. 54, n. 12, p. -, 2021.
Web of Science Citations: 0
Abstract

Due to the high transfusion volume, polytransfused patients with sickle cell disease (SCD) and beta-thalassemia are constantly exposed to parenterally transmitted infections. Currently, we have little information about the virome of such patients and how the virological composition might be influenced by the hemotherapy procedures that these patients receive. The objective of this study was to compare the viral diversity between these two groups with respect to the viral abundance and how it might be affected by the specific conditions of these groups. We sequenced by next-generation sequencing (NGS) and compared the virome of 30 patients with beta-thalassemia major, 45 with SCD, and 16 blood donors from the Blood Center of Ribeirão Preto, Brazil. Predominantly, commensal viruses including Torque teno virus (TTV) genotypes and human pegiviris-1 (HPgV-1) were identified in each group. Strikingly, while HPgV-1 reads were dominant in the SCD group, thalassemic patients showed high TTV abundance, expressed both in viral reads and genotypes. We speculated that the commensal virome of polytransfused patients might be influenced by the transfusion frequency and disease characteristics and that commensal viruses might be used as important genetic biomarkers for these hematological disturbances. Nevertheless, more specific studies are necessary to confirm a relationship between blood virome and transfusion treatment. (AU)

FAPESP's process: 19/07861-8 - Development of a bioinformatic pipeline for identification of emerging infectious diseases among patients in regimen of chronic transfusion
Grantee:Ian Nunes Valença
Support type: Scholarships in Brazil - Master
FAPESP's process: 20/13194-1 - Evaluation of the prevalence and application of next generation sequencing methods for identification of emerging viruses in the area of hemotherapy
Grantee:Victória Simionatto Zucherato
Support type: Scholarships in Brazil - Technical Training Program - Technical Training
FAPESP's process: 19/22155-2 - Cellular characterization of hematopoietic niches in murine model of sickle cell anemia
Grantee:Felipe Augusto Rós
Support type: Scholarships in Brazil - Master
FAPESP's process: 17/23205-8 - Evaluation of the impact of emerging and reemerging viruses in the field of hemotherapy and stem cell transplantation by multiple-research approach
Grantee:Svetoslav Nanev Slavov
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 18/15826-5 - Evaluation of the impact of emerging and reemerging viruses in the field of hemotherapy and stem-cell transplantation by multiple-research approach
Grantee:Svetoslav Nanev Slavov
Support type: Scholarships in Brazil - Young Researchers