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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Luminescent imaging of insulin amyloid aggregation using a sensitive ruthenium-based probe in the red region

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Pereira, Lorena M. B. [1] ; Cali, Mariana P. [1] ; Marchi, Rafael C. [1] ; Pazin, Wallance M. [2] ; Carlos, Rose M. [1]
Total Authors: 5
[1] Univ Fed Sao Carlos, Dept Chem, BR-13565905 Sao Carlos, SP - Brazil
[2] Univ Estadual Paulista, Dept Phys, BR-19060900 Presidente Prudente, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of Inorganic Biochemistry; v. 224, NOV 2021.
Web of Science Citations: 0

A sensitive and selective strategy to identify insulin fibrils remains a challenge for researchers in amyloid protein research. Thus, it is critical to detect, in vitro, the species generated during amyloid aggregation, particularly the fibrillar species. Here we demonstrate that the luminescent complex cis-{[}Ru(phen)(2)(3,4Apy)(2)](2+) (RuApy; phen = 1,10-phenanthroline; 3,4Apy = 3,4-diaminopyridine) is a rapid, low-cost alternative to in vitro detection of fibrillar insulin, using conventional optical techniques. The RuApy complex displays emission intensity enhancement at 655 nm when associated with insulin, which enables imaging of the conformational changes of the protein's self-aggregation. The complex shows high sensitivity to fibrillar insulin with a limit of detection of 0.85 mu M and binding affinity of 12.40 +/- 1.84 mu M which is comparable to those of Thioflavin T and Congo red, with the advantage of minimizing background fluorescence, absorption of light by biomolecules, and light scattering from physiologic salts in the medium. (AU)

FAPESP's process: 20/12129-1 - Immunoassay for COVID-19 diagnosis based on surface-enhanced Raman scattering technique
Grantee:Wallance Moreira Pazin
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 19/21143-0 - Amyloid protein aggregates and the relationship between Alzheimer's Disease and type 2 Diabetes investigated by Ru(II) luminescent complexes
Grantee:Rose Maria Carlos
Support Opportunities: Regular Research Grants
FAPESP's process: 17/00839-1 - Analysis of the structure-toxicity relationship of b-amyloid peptides and neuroprotective effect of luminescent Ru (II) complexes
Grantee:Rose Maria Carlos
Support Opportunities: Regular Research Grants