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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Inositol monophosphatase 1 (IMPA1) mutation in intellectual disability patients impairs neurogenesis but not gliogenesis

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Figueiredo, Thalita [1, 2, 3] ; Mendes, Ana P. D. [2] ; Moreira, Danielle P. [1] ; Goulart, Ernesto [1] ; Oliveira, Danyllo [1] ; Kobayashi, Gerson S. [1] ; Stern, Shani [2, 4] ; Kok, Fernando [1] ; Marchetto, Maria C. [2] ; Santos, Renata [2, 5] ; Gage, Fred H. [2] ; Zatz, Mayana [1]
Total Authors: 12
[1] Univ Sao Paulo, Human Genome & Stem Cell Res Ctr, Biosci Inst, 106 Rua Matao, BR-05508090 Sao Paulo - Brazil
[2] Salk Inst Biol Studies, Lab Genet, 10010 North Torrey Pines Rd, La Jolla, CA 92037 - USA
[3] Univ Fed Alagoas, Fac Med, BR-57072900 Maceio, Alagoas - Brazil
[4] Univ Haifa, Fac Nat Sci, Sagol Dept Neurobiol, IL-3498838 Haifa - Israel
[5] Univ Paris, IPNP, INSERM U1266, Lab Dynam Neuronal Struct Hlth & Dis, 102 Rue Sante, F-75014 Paris - France
Total Affiliations: 5
Document type: Journal article
Source: MOLECULAR PSYCHIATRY; v. 26, n. 7, p. 3558-3571, JUL 2021.
Web of Science Citations: 0

A homozygous mutation in the inositol monophosphatase 1 (IMPA1) gene was recently identified in nine individuals with severe intellectual disability (ID) and disruptive behavior. These individuals belong to the same family from Northeastern Brazil, which has 28 consanguineous marriages and 59 genotyped family members. IMPA1 is responsible for the generation of free inositol from de novo biosynthesis and recycling from inositol polyphosphates and participates in the phosphatidylinositol signaling pathway. To understand the role of IMPA1 deficiency in ID, we generated induced pluripotent stem cells (iPSCs) from patients and neurotypical controls and differentiated these into hippocampal dentate gyrus-like neurons and astrocytes. IMPA1-deficient neuronal progenitor cells (NPCs) revealed substantial deficits in proliferation and neurogenic potential. At low passage NPCs (P1 to P3), we observed cell cycle arrest, apoptosis, progressive change to a glial morphology and reduction in neuronal differentiation. These observations were validated by rescuing the phenotype with myo-inositol supplemented media during differentiation of patient-derived iPSCs into neurons and by the reduction of neurogenic potential in control NPCs-expressing shIMPA1. Transcriptome analysis showed that NPCs and neurons derived from ID patients have extensive deregulation of gene expression affecting pathways necessary for neurogenesis and upregulation of gliogenic genes. IMPA1 deficiency did not affect cell cycle progression or survival in iPSCs and glial progenitor cells or astrocyte differentiation. Therefore, this study shows that theIMPA1mutation specifically affects NPC survival and neuronal differentiation. (AU)

FAPESP's process: 16/09618-5 - What is the role of IMPA1 enzyme in Familial Intellectual Disability?
Grantee:Thalita Cristina Figueiredo Cunha
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 17/19877-0 - Understanding the role of IMPA1 mutation in hippocampal neurons derived from patients with familial intellectual disability
Grantee:Thalita Cristina Figueiredo Cunha
Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
FAPESP's process: 13/08028-1 - CEGH-CEL - Human Genome and Stem Cell Research Center
Grantee:Mayana Zatz
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 19/18469-1 - Development of universal hepatic organoids produced from IPS cells
Grantee:Ernesto da Silveira Goulart Guimarães
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 14/50931-3 - Aging and genetic disorders: genomics and metagenomics
Grantee:Mayana Zatz
Support Opportunities: Research Projects - Thematic Grants