Gimenez, Alba Marina
Salman, Ahmed M.
Marques, Rodolfo F.
Kim, Young Chan
Janse, Chris J.
Soares, Irene S.
Total Authors: 9
 Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, Sao Paulo, SP - Brazil
 Univ Oxford, Jenner Inst, Nuffield Dept Med, Henry Wellcome Bldg Mol Physiol, Roosevelt Dr, Oxford OX3 7BN - England
 Leiden Univ Med Ctr, Dept Parasitol, Leiden Malaria Res Grp, Ctr Infect Dis, L4-Q, Albinusdreef 2, NL-2333 ZA Leiden - Netherlands
 Inst Politecnico Nacl, Unidad Adolfo Lopez Mateos, Ave Luis Enrique Erro S-N, Mexico City 07738, DF - Mexico
Total Affiliations: 4
SEP 9 2021.
Web of Science Citations:
Malaria is a highly prevalent parasitic disease in regions with tropical and subtropical climates worldwide. Among the species of Plasmodium causing human malaria, P. vivax is the second most prevalent and the most geographically widespread species. A major target of a pre-erythrocytic vaccine is the P. vivax circumsporozoite protein (PvCSP). In previous studies, we fused two recombinant proteins representing three allelic variants of PvCSP (VK210, VK247 and P. vivax-like) to the mumps virus nucleocapsid protein to enhance immune responses against PvCSP. The objective of the present study was to evaluate the protective efficacy of these recombinants in mice challenged with transgenic P. berghei parasites expressing PvCSP allelic variants. Formulations containing Poly (I:C) or Montanide ISA720 as adjuvants elicited high and long-lasting IgG antibody titers specific to each PvCSP allelic variant. Immunized mice were challenged with two existing chimeric P. berghei parasite lines expressing PvCSP-VK210 and PvCSP-VK247. We also developed a novel chimeric line expressing the third allelic variant, PvCSP-P. vivax-like, as a new murine immunization-challenge model. Our formulations conferred partial protection (significant delay in the time to reach 1% parasitemia) against challenge with the three chimeric parasites. Our results provide insights into the development of a vaccine targeting multiple strains of P. vivax. (AU)