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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Protoporphyrin IX (PpIX) loaded PLGA nanoparticles for topical Photodynamic Therapy of melanoma cells

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Author(s):
da Silva, Darlan Barbosa [1] ; da Silva, Carolina Loureiro [1] ; Davanzo, Nathalia Nossi [2] ; Souza, Rodrigo da Silva [3] ; Correa, Rodrigo Jose [3] ; Tedesco, Antonio Claudio [2] ; Riemma Pierre, Maria Bernadete [1]
Total Authors: 7
Affiliation:
[1] Univ Fed Rio de Janeiro, Sch Pharm, Av Carlos Chagas Filho 373, BR-21941902 Rio De Janeiro, RJ - Brazil
[2] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Ctr Nanotechnol & Tissue Engn, Dept Chem, Photobiol & Photomed Res Grp, Av Bandeirantes 3900, BR-14040901 Vila Monte Alegre - Brazil
[3] Univ Fed Rio de Janeiro, Inst Quim, Rio De Janeiro - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Photodiagnosis and Photodynamic Therapy; v. 35, SEP 2021.
Web of Science Citations: 0
Abstract

Introduction: Nanoparticles (Np) can increase drug efficacy and overcome problems associated with solubility and aggregation in a solution of PpIX. Purpose: Evaluate if Np interferes in the photophysical and photobiological capacity of the PpIX comparing with free PpIX intended for topical PDT of melanoma. Methods: In vitro photophysical evaluation of Np-PpIX was carried out through singlet oxygen (O-1(2)) quantum yield. In vitro cytotoxicity and phototoxicity assays have used murine melanoma cell culture. Results: The quantum yield of singlet oxygen has shown that Np did not influence the formation capacity of this reactive species. In the dark, all PpIX-Nps concentrations were less cytotoxic compared to free drugs. At a higher light dose (1500 mJ.cm(2)) 3.91 mu g/mL PpIX had similar % viable cells for free and Np (similar to 34 %) meaning Nps did not interfere in the photodynamic effect of PpIX. However, at 7.91 mu g/mL the phototoxicity increased for both (5.8 % viable cells for free versus 21.7 % for Nps). Despite the higher phototoxicity of free PpIX at this concentration, greater cytotoxicity in the dark was obtained (similar to 49 % viable cells for free versus similar to 90 .6 % Np) which means Nps protect the tumor tissue from the photodynamic action of PpIX. Conclusions: Np is a potential delivery system for melanoma skin cancer, since it maintained the photophysical properties of PpIX and excellent in vitro phototoxicity effect against melanoma cells, reducing cell viability similar to 80 % (7.91 mu g/mL PpIX in Nps) and provides safe PDT (due to lower cytotoxicity in the dark). (AU)

FAPESP's process: 13/50181-1 - Use of drugs containing nanocarriers with photosensitizers and/or other active compounds applied to cell therapy and treatment of central nervous system disorders
Grantee:Antonio Claudio Tedesco
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 15/16660-5 - Analysis of the nanoemulsions associated with photodynamic therapy in the treatment of breast cancer
Grantee:Marilia de Freitas Calmon
Support Opportunities: Regular Research Grants