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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of curcumin-mediated antimicrobial photodynamic therapy associated to different chelators against Enterococcus faecalis biofilms

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Author(s):
Cusicanqui Mendez, Daniela Alejandra [1] ; Cardenas Cuellar, Maricel Rosario [2] ; Pedrinha, Victor Feliz [3] ; Velasquez Espedilla, Evelyn Giuliana [2] ; de Andrade, Flaviana Bombarda [2] ; Rodrigues, Patricia de Almeida [3] ; Cruvinel, Thiago [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Bauru Sch Dent, Dept Pediat Dent Orthodont & Publ Hlth, Alameda Dr Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil
[2] Univ Sao Paulo, Bauru Sch Dent, Dept Operat Dent Endodont & Dent Mat, Bauru, SP - Brazil
[3] Fed Univ Para, Sch Dent, Lab Dent Mat, Grad Program Dent, Belem, Para - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Photodiagnosis and Photodynamic Therapy; v. 35, SEP 2021.
Web of Science Citations: 0
Abstract

Background: The aim of this study was to evaluate curcumin-mediated antimicrobial photodynamic therapy (aPDT) action combined or not with ethylenediaminetetraacetic acid (EDTA) and hydroxyethylidene bisphosphonate (HEBP) on Enterococcus faecalis biofilms. Methods: Enterococcus faecalis biofilms were grown on dentin bovine discs in brain heart infusion (BHI) medium with 1% glucose, in aerobic conditions at 37 degrees C for 7 days. Then, they were randomly distributed to one of experimental conditions, as follows: control, 75 J.cm- 2 LED, 600 Irmol.L- 1 curcumin, 17% EDTA, 18% HEBP, 600 Irmol.L-1 curcumin plus 75 J.cm- 2 LED, 600 Irmol.L- 1 curcumin plus 17% EDTA, 600 Irmol.L-1 curcumin plus 18% HEBP, 600 Irmol.L-1 curcumin plus 17% EDTA and 75 J.cm- 2 LED or 600 Irmol.L-1 curcumin plus 18% HEBP and 75 J.cm- 2 LED. The viability of microorganisms and the vitality of biofilms were determined by colony forming unit counts and confocal scanning laser microscopy (CSLM), respectively. Statistical analysis was conducted by Kruskal Wallis and Dunn's post-hoc tests (alpha = 0.05). Results: The results showed that all combinations of aPDT with chelators significantly reduced the viability of microbial cells and the vitality of biofilms in comparison to control, even when considering deeper layers of biofilms. Conclusion: The combination of curcumin with EDTA and HEBP similarly improved the effect of aPDT on E. faecalis biofilms. (AU)

FAPESP's process: 14/10897-0 - Effect of antimicrobial photodynamic therapy on dentin caries microcosms
Grantee:Thiago Cruvinel da Silva
Support Opportunities: Regular Research Grants
FAPESP's process: 14/22238-1 - Effect of antimicrobial photodynamic therapy on dentin caries microcosms
Grantee:Eliézer Gutierres
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 15/08595-9 - Effect of antimicrobial photodynamic therapy on specific microorganisms from dentin caries microcosms
Grantee:Daniela Alejandra Cusicanqui Méndez
Support Opportunities: Scholarships in Brazil - Master