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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Hormetic effects of low-dose gamma rays in soybean seeds and seedlings: A detection technique using optical sensors

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Author(s):
Oliveira, Nielsen Moreira [1] ; de Medeiros, Andre Dantas [2] ; Nogueira, Marina de Lima [3] ; Arthur, Valter [4] ; Mastrangelo, Thiago de Araujo [4] ; da Silva, Clissia Barboza [4]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Coll Agr Luiz de Queiroz, Dept Crop Sci, BR-13418900 Piracicaba, SP - Brazil
[2] Univ Fed Vicosa, Dept Agron, BR-36570900 Vicosa, MG - Brazil
[3] Univ Sao Paulo, Coll Agr Luiz de Queiroz, Dept Genet, BR-13418900 Piracicaba, SP - Brazil
[4] Univ Sao Paulo, Ctr Nucl Energy Agr, BR-13416000 Piracicaba, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: COMPUTERS AND ELECTRONICS IN AGRICULTURE; v. 187, AUG 2021.
Web of Science Citations: 0
Abstract

The use of radiation technology in agriculture has increased remarkably in recent years. The stimulating effect of low-dose gamma rays, known as hormesis, improves the adaptive response of living organisms to more severe challenges. Different areas of agriculture such as plant physiology, crop breeding and seed biology demand accurate methods for real-time diagnosis of the ionizing radiation effect on biological systems. However, traditional measures are still calculated from slow, laborious and destructive techniques, which depend on human vision. Here, we present two robust optical sensors based on multispectral and fluorescence imaging approaches for rapid and non-destructive detection of the hormetic effects of low-dose gamma radiation in soybean seeds and seedings. Multispectral images were acquired from seeds treated with gamma radiation (0, 12, 16 and 20 Gy), and the reflectance, autofluorescence, texture and color features were extracted from images. Next, a chlorophyll fluorescence-based technique was applied to measure the minimal level of fluorescence (F-0), maximum fluorescence (F-M), average chlorophyll a fluorescence and quantum yield of photosystem II (F-V/F-M) in seedlings grown from irradiated and non-irradiated seeds. Using a reflectance technology, the seedlings were also evaluated for anthocyanin, chlorophyll a and normalized difference vegetation index (NDVI). Results showed that multispectral and fluorescence-based techniques provide useful information on the hormetic effects of gamma rays in soybean seeds and seedlings, which are invisible to the naked eye. Our findings provide a better understanding of the link between spectral and fluorescence parameters and the hormetic effect of low-dose gamma rays. From a practical point of view, such optical sensors can provide markers for fast, non-destructive and reliable identification of the best gamma-ray doses to stimulate soybean growth parameters. However, we strongly support in-depth studies of the dose-response relationship in seeds with different initial physiological potential from different soybean genotypes. (AU)

FAPESP's process: 18/03802-4 - Multi-user equipment approved in grant 2017/15220-7: imaging system VideoMeterLab
Grantee:Clíssia Barboza da Silva
Support type: Multi-user Equipment Program
FAPESP's process: 18/01774-3 - Non-destructive image analysis methods for seed quality evaluation
Grantee:Clíssia Barboza da Silva
Support type: Scholarships in Brazil - Young Researchers
FAPESP's process: 18/03793-5 - Multi-user equipment approved in grant 2017/15220-7: imaging system SeedReporter camera spectral & colour
Grantee:Clíssia Barboza da Silva
Support type: Multi-user Equipment Program
FAPESP's process: 17/15220-7 - Non-destructive image analysis methods for seed quality evaluation
Grantee:Clíssia Barboza da Silva
Support type: Research Grants - Young Investigators Grants