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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effect of uterine ozone therapy and anticoagulant sampling on oxidative stress parameters in mares

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Martins de Almeida, Breno Fernando [1] ; Amatti, Lidiana Zanetti [1] ; de Souza, Giovanna Gati [1] ; Garcia, Luana Venancio [1] ; Montechiesi, Daniela Fernandez [1] ; Ignacio, Fernanda Saules [2] ; de Oliveira, Paula Lima [3] ; Costa, Leticia Ramos [3] ; Floriano, Beatriz Perez [1] ; Melo Bosculo, Maria Rachel [3] ; Fernandes Joaquim, Jean Guilherme [4] ; Peres Rubio, Camila [5]
Total Authors: 12
[1] Univ Ctr Integrated Fac Ourinhos Unifio, Dept Vet Med, Ourinhos, SP - Brazil
[2] Fac Eduvale, Dept Vet Med, Avare, SP - Brazil
[3] Univ Ctr Integrated Fac Ourinhos Unifio, Vet Teaching Hosp Roque Quagliato, Ourinhos, SP - Brazil
[4] Inst Bioethicus, Sao Paulo, SP - Brazil
[5] Univ Murcia, Fac Vet Med, Dept Anim Med & Surg, Reg Campus Int Excellence Campus Mare Nostrum, Murcia - Spain
Total Affiliations: 5
Document type: Journal article
Source: Research in Veterinary Science; v. 136, p. 503-511, MAY 2021.
Web of Science Citations: 0

With the purpose of assessing the effects of uterine ozone therapy and anticoagulant sampling on oxidative stress (OS) parameters in mares, ten mares underwent three consecutive days of uterine ozone therapy by flushing the uterus with ozonated lactated Ringer's solution followed by insufflation with ozone-oxygen gas. Serum samples were obtained at baseline and days 3, 6, 10 and 17 to determine the effect of ozone therapy on OS markers. Plasma obtained with anticoagulants citrate, ethylenediaminetetraacetic acid (EDTA) and heparin were at baseline and 6 days following therapy to determine the effect of anticoagulant on OS parameters. Antioxidants albumin and uric acid, total antioxidant capacity (TAC) using four different methods, total oxidant capacity (TOC) and lipid peroxidation were determined through photocolorimetry. Statistical analyses comprised repeated measures ANOVA followed by Dunnett's test or Friedman followed by Dunn's post-hoc test. Differences were considered significant when p < 0.05. Uterine ozone therapy significantly decreased uric acid, TAC in all four different methods, concomitantly with an increase on TOC at days 3 and 6 following therapy. No changes were observed on albumin and lipid peroxidation levels. Anticoagulants prevented the detection of oxidative stress induced by uterine ozone therapy depending on the method of analysis. In conclusion, uterine ozone therapy causes systemic oxidative stress in mares and the choice of anticoagulant sampling interferes with laboratory tests. (AU)

FAPESP's process: 20/03024-1 - Effect of uterine ozone therapy and anticoagulant sampling on oxidative stress parameters in mares
Grantee:Lidiana Zanetti Amatti
Support Opportunities: Scholarships in Brazil - Scientific Initiation