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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

IRST REPORT OF GENUS Cryptosporidium IN CERVIDS SPECIES: Mazama americana, Mazama nana AND Blastocerus dichotomu

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Pires Teixeira, Weslen Fabricio [1] ; de Oliveira, Marcio Leite [2] ; de Faria Peres, Pedro Henrique [2] ; Miranda Oliveira, Bruno Cesar [3] ; Nagata, Walter Bertequini [3] ; Vieira, Dielson da Silva [4] ; de Andrade Junior, Anassilton Moreira [1] ; Ferrari, Elis Domingos [3] ; Barbanti Duarte, Jose Mauricio [2] ; Meireles, Marcelo Vasconcelos [4] ; Zanetti Lopes, Welber Daniel [1] ; Saraiva Bresciani, Katia Denise [3]
Total Authors: 12
[1] Fed Univ Goias UFG, Dept Vet Med, Goiania, Go - Brazil
[2] Sao Paulo State Univ UNESP, Sch Agr & Vet Sci, Sao Paulo - Brazil
[3] Sao Paulo State Univ Unesp, Sch Vet Med, Dept Support Prod & Anim Hlth, Sao Paulo - Brazil
[4] Sao Paulo State Univ UNESP, Sch Vet Med, Dept Clin Surg & Anim Reprod, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Web of Science Citations: 0

We analyzed Cryptosporidium spp. in fecal samples of wild cervids (Ozotoceros bezoarticus, Blastocerus dichotomus, Mazama nana, Mazama americana, and Mazama bororo) from many Brazilian regions, a fact unprecedented in the literature. Sniffer dogs were used to collect 936 fecal samples of cervids from 14 Brazilian localities. Cervids species were identified using polymerase chain reaction (PCR) performed from genomic DNA extracted from 563 fecal samples of Ozotoceros bezoarticus, Blastocerus dichotomus, Mazama nana, Mazama americana, and Mazama bororo. Cryptosporidium spp. oocyst screening was performed using malachite green negative staining. Nested PCR (nPCR) protocols targeting the 18S rRNA and GP60 genes followed by genetic sequencing were performed for Cryptosporidium spp. detection and Cryptosporidium parvum subtyping, respectively. Nested PCR targeting actin gene and genetic sequencing were performed in samples with non-identified Cryptosporidium species by 18S rRNA amplicon sequencing. The association between the occurrence of Cryptosporidium and the presence of bovines in the same locality was evaluated using Fisher's exact test. The positivity rates of diagnostic methods were compared by McNemar test and the Kappa correlation coefficient. The prevalence rates of Cryptosporidium spp. in cervids were 1.42% (8/563) and 0.36% (2/563) by nPCR and malachite green negative staining, respectively. C. parvum IIaA16G3R1 isolate was identified in three fecal samples from M. americana, two from M. nana and one from B. dichotomus. Cryptosporidium ryanae were found in one sample from B. dichotomus. We identified a new Cryptosporidium genotype, named Cryptosporidium deer genotype BR, from one M. americana fecal sample. (AU)

FAPESP's process: 10/50748-3 - The gray brockets in Brazil (Mammalia; Cervidae; Mazama): detection of the genetic, morphologic and ecologic variants to explain the complex evolution in this group
Grantee:José Maurício Barbanti Duarte
Support type: Research Projects - Thematic Grants
FAPESP's process: 15/10086-5 - Occurrence and molecular characterization of Cryptosporidium spp. in Brazilian wildlife deer
Grantee:Weslen Fabricio Pires Teixeira
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 12/50206-1 - Dwarf brocket deer (Mazama nana) distribution and population estimates using non-invasive genetics sampling and modeling
Grantee:José Maurício Barbanti Duarte
Support type: Regular Research Grants
FAPESP's process: 15/25742-5 - Distribution and density of Mazama genus deer species at the Atlantic Forest
Grantee:Márcio Leite de Oliveira
Support type: Scholarships in Brazil - Post-Doctorate