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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

erivascular clusters of Th2 cells and M2 macrophages in allergic contact dermatitis to methylchloroisothiazolinone and methylisothiazolinon

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Virgens, Anangelica R. [1, 2] ; Goes, Heliana F. O. [1, 2] ; de Carvalho, Gabriel C. [2] ; Pietrobon, Anna Julia [2] ; C. C. Branco, Anna Claudia [2] ; Ramos, Yasmim A. L. [3] ; Pereira, Naiura V. [1] ; Orfali, Raquel L. [1] ; Aoki, Valeria [1] ; da Silva, Luiz Fernando F. [3] ; Sotto, Mirian N. [3] ; dos Reis, Vitor M. S. [1] ; Sato, Maria N. [2]
Total Authors: 13
[1] Univ Sao Paulo, Sch Med, Dept Dermatol, Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Sch Med, Trop Med Inst Sao Paulo, Dept Dermatol, Lab Invest Med, LIM 56, LIM-56, Av Dr Eneas Carvalho Aguiar 470, 3rd Floor, BR-05403000 Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Sch Med, Dept Pathol, Sao Paulo, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: EXPERIMENTAL DERMATOLOGY; v. 31, n. 2 AUG 2021.
Web of Science Citations: 0

Background Methylisothiazolinone (MI) and Methylchloroisothiazolinone (MCI) are among the most common skin sensitizers, yet the immunological events that occur during MCI/MI allergic contact dermatitis (ACD) are still poorly understood. Objectives: To analyse dendrocytes, macrophage subtypes and T cells in skin during the elicitation phase of MCI/MI ACD. Methods Thirteen patients with positive patch test reactions to MCI/MI (ACD group) and 11 individuals with negative patch test results were selected. Skin biopsies were only performed at 48 hours of patch testing. Immunohistochemistry was conducted to assess T cells, dendrocytes (Factor XIIIa), M1 (p-Stat1, CD68) and M2 (c-Maf, CD163) macrophages. Transcriptional analyses were performed for cytokines and related factors, and further compared to atopic dermatitis samples (n=4). Immunofluorescence assays addressed T cells location, along with IL-4 or IL-13, within the skin. Results MCI/MI elicited dermal dendrocytes and macrophages, pronouncedly the M2 subtype. T cells, majorly CD4+ T cells, accumulated in the perivascular areas. Similarly, abundant IL-4 protein was detected in these areas. There was an upregulation of IL-4 and IL-13 mRNA expression, a mild increase in IFNG mRNA levels and a down-regulation of RORC in the ACD group. Immunofluorescence revealed dermal clusters of T cells co-localized with IL-4. Conclusions M2 macrophages and Th2 cells participate in the immunopathogenesis of MCI/MI ACD. Dermal dendrocytes and M2 macrophages may assist the formation of CD4+ T cells perivascular clusters. These findings render a mechanistic insight into the MCI/MI reaction. Further analysis at different timepoints of patch testing is required to fully comprehend this ACD kinetics. (AU)

FAPESP's process: 17/12475-4 - Analysis of the pattern recognition receptors and crosstalk between dendritic cells-T lymphocytes and macrophages in allergic contact dermatitis by methylisothiazolinone and methylchloroisothiazolinone
Grantee:Vitor Manoel Silva dos Reis
Support Opportunities: Regular Research Grants