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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Multitoxin immunoaffinity analysis of aflatoxins and ochratoxin A in spices

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Iha, Maria Helena [1] ; Rodrigues, Matheus Leandro [1] ; Trucksess, Mary Wat [2]
Total Authors: 3
[1] Adolfo Lutz Inst Ribeirao Preto VI, Ctr Chem Sci & Bromatol, Rua Minas 877, BR-14085410 Ribeirao Preto, SP - Brazil
[2] US FDA, Ctr Food Safety & Appl Nutr, Off Regulatory Sci, College Pk, MD - USA
Total Affiliations: 2
Document type: Journal article
Source: JOURNAL OF FOOD SAFETY; v. 41, n. 5 AUG 2021.
Web of Science Citations: 0

A multitoxin method for aflatoxins (AFs) and ochratoxin A (OTA) was modified and validated to analyze black pepper, colorifico, nutmeg, paprika, and turmeric. The method consisted of acetonitrile, NaHCO3 and methanol extraction solution, multimycotoxin immunoaffinity column purification and isolation, reversed-phase liquid chromatography separation, and fluorescence detection. The limit of detection was <0.1 ng/g for AFs and OTA. Mean recoveries of added AFs ranged from 68 to 88% with relative standard deviation of repeatability (RSDr) values <15%; aflatoxin B-1 (AFB(1)) from 69 to 92% with RSDr <17%; and OTA from 75 to 110% with RSDr <10% at spiked levels ranged from 8 to 40 ng/g for AFs, 4 to 20 ng/g for AFB(1), and 4 to 20 ng/g for OTA, respectively. Recoveries of added aflatoxin G(2) were approximately 50% at spiked levels ranged from 1 to 5 ng/g. Recoveries of aflatoxin B-2 and aflatoxin G(1) were >70%. The linearity of the method for AFs ranged from 0.4 to 100 ng/g, for OTA from 0.2 to 40 ng/g with R-2 values exceeding 0.99. Results of a single laboratory validation study validated the performance of this method meeting the method acceptability criteria. No differences in toxin detection were determined in robustness testing in which minor changes were introduced to the method. (AU)

FAPESP's process: 18/07009-7 - Quality of spices commercialized in the São Paulo State: physico-chemical (incidence of mycotoxins, analysis of artificial dyes) and microscopic (identification of histological elements, investigation of extraneous materials)
Grantee:Maria Helena Iha Matias
Support Opportunities: Regular Research Grants