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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Celecoxib treatment dampens LPS-induced periapical bone resorption in a mouse model

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Petean, I. B. F. [1] ; Almeida-Junior, L. A. [1] ; Arnez, M. F. M. [1] ; Queiroz, A. M. [1] ; Silva, R. A. B. [1] ; Silva, L. A. B. [1] ; Faccioli, L. H. [2] ; Paula-Silva, F. W. G. [1, 2]
Total Authors: 8
[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Pediat Dent, Ave Cafe S-N, BR-14040904 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, Lab Inflamacao & Imunol Parasitoses, Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: International Endodontic Journal; v. 54, n. 8, p. 1289-1299, AUG 2021.
Web of Science Citations: 1

Aim To evaluate the efficacy of selective and nonselective inhibitors of cyclooxygenase-2 enzymes in the treatment of experimental apical periodontitis induced by bacterial lipopolysaccharide (LPS) in vivo in a mouse model. Methodology Thirty-six C57BL/6 mice were used. After access cavity preparation, a solution containing E. coli LPS (1.0 mu g mu L-1) was inoculated into the root canals of the mandibular and maxillary right first molars (n = 72) After 30 days, apical periodontitis was established and the animals were systemically treated with celecoxib, a selective COX-2 inhibitor (15 mg kg(-1)), or indomethacin, a nonselective COX-2 inhibitor (5 mg kg(-1)), for 7 and 14 days. Blocks containing teeth and bone were removed for histopathological and histometric analyses (haematoxylin and eosin), evaluation of osteoclasts numbers (tartrate-resistant acid phosphatase enzyme - TRAP) and immunohistochemistry for RANK, RANKL and OPG. Gene expression was performed using reverse transcription and real-time polymerase chain reaction (qRT-PCR) for RANK, RANKL, OPG, TRAP, MMP-9, cathepsin K and calcitonin receptor. Histopathological, histometric, TRAP, immunohistochemistry and qRT-PCR data were evaluated using Kruskal-Wallis followed by Dunn's test (alpha = 0.05). Results Systemic administration of celecoxib for 7 and 14 days prevented periapical bone resorption (P < 0.0001), differently from indomethacin that exacerbated bone resorption at 7 days (P < 0.0001) or exerted no effect at 14 days (P = 0.8488). Celecoxib treatment reduced osteoclast formation in apical periodontitis, regardless of the period of treatment (P P = 0.026 for 14 days). Administration of celecoxib or indomethacin differentially modulated the expression of genes involved in bone resorption. At 7 days, celecoxib and indomethacin treatment significantly inhibited expression of mRNA for cathepsin K (P = 0.0005 and P = 0.016, respectively) without changing TRAP, MMP-9 and calcitonin receptor gene expression. At 14 days, celecoxib significantly inhibited expression of mRNA for MMP-9 (P < 0.0001) and calcitonin receptor (P = 0.004), whilst indomethacin exerted no effect on MMP-9 (P = 0.216) and calcitonin receptor (P = 0.971) but significantly augmented cathepsin K gene expression (P = 0.001). Conclusions The selective COX-2 inhibitor celecoxib reduced osteoclastogenic signalling and activity that dampened bone resorption in LPS-induced apical periodontitis in mice, with greater efficacy than the nonselective inhibitor indomethacin. (AU)

FAPESP's process: 10/17611-4 - Mechanisms involved in the regulation of 5-lipoxygenase pathway in experimentally-induced apical periodontitis
Grantee:Francisco Wanderley Garcia de Paula e Silva
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 13/09595-7 - The role of selective and non-selective inhibitors enzyme cyclooxygenase-2 and 5-lipoxygenase enzyme in osteoclastogenesis after the development of experimental periapical
Grantee:Igor Bassi Ferreira Petean
Support Opportunities: Scholarships in Brazil - Scientific Initiation