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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Osteoclast formation, inflammation, and matrix metalloproteinase-9 are downregulated in bone repair following root canal treatment in dogs teeth

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Garcia Paula-Silva, Francisco Wanderley [1] ; Manfrin Arnez, Maya Fernanda [1] ; Chaves Lamarque, Giuliana de Campos [1] ; Petille, Renato [1] ; Ribeiro-Santos, Fernanda Regina [1, 2] ; de Sena, Marina Fernandes [1] ; Nelson-Filho, Paulo [1] ; Bezerra da Silva, Lea Assed [1]
Total Authors: 8
[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Pediat Clin, Ribeirao Preto, SP - Brazil
[2] Univ Pernambuco, Arco Verde, PE - Brazil
Total Affiliations: 2
Document type: Journal article
Source: CLINICAL ORAL INVESTIGATIONS; v. 25, n. 7, p. 4699-4707, JUL 2021.
Web of Science Citations: 0

Objectives The aim of this study was to investigate the inflammatory infiltrate, osteoclast formation, and expression of MMP-9 during the healing phase following root canal treatment in teeth with apical periodontitis. Materials and methods Apical periodontitis was induced in dogs teeth, and root canal treatment was performed in a single visit or using calcium hydroxide as intracanal medication. One hundred and eighty days following treatment the presence of inflammation was examined, and the tissues were stained to detect osteoclasts by means of a tartrate resistant alkaline phosphatase (TRAP) assay. Synthesis of MMP-9 was detected using Western blotting and immunohistochemistry. Results Teeth with apical periodontitis that had root canal therapy performed in a single visit presented a higher synthesis of MMP-9 compared with root canal treatment using calcium hydroxide. Treatment with calcium hydroxide resulted in a reduced amount of inflammatory cells and MMP-9 positive cells. Osteoclast formation, the number of MMP-9 positive osteoclasts and cementocytes, was reduced following root canal treatment, regardless of the root canal treatment protocol used. Conclusion Root canal treatment reduced the amount of inflammatory cells and osteoclasts in periapical area. The use of calcium hydroxide as intracanal medication resulted in a lower synthesis of MMP-9, though the number of osteoclasts and MMP-9 positive osteoclasts were similar between the groups. (AU)

FAPESP's process: 09/16882-7 - Osteoclastogenesis and matrix metalloproteinase-9 expression in apical and periapical repair following root canal treatment
Grantee:Renato Petille
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 19/02060-7 - American Academy of Pediatric Dentistry 72nd Annual Session
Grantee:Francisco Wanderley Garcia de Paula e Silva
Support type: Research Grants - Meeting - Abroad