Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Highly Dynamic Polynuclear Metal Cluster Revealed in a Single Metallothionein Molecule

Full text
Author(s):
Yuan, Guodong [1] ; Curtolo, Felipe [2] ; Deng, Yibing [1] ; Wu, Tao [1] ; Tian, Fang [1] ; Ma, Qun [1] ; Liu, Yutong [1] ; Zuo, Jinglin [1] ; Arantes, Guilherme Menegon [2] ; Zheng, Peng [1]
Total Authors: 10
Affiliation:
[1] Nanjing Univ, Sch Chem & Chem Engn, Chem & Biomed Innovat Ctr ChemBIC, State Key Lab Coordinat Chem, Nanjing 210023 - Peoples R China
[2] Univ Sao Paulo, Inst Quim, Dept Biochem, Ave Prof Lineu Prestes 748, BR-05508900 Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: RESEARCH; v. 2021, JUL 14 2021.
Web of Science Citations: 0
Abstract

Human metallothionein (MT) is a small-size yet efficient metal-binding protein, playing an essential role in metal homeostasis and heavy metal detoxification. MT contains two domains, each forming a polynuclear metal cluster with an exquisite hexatomic ring structure. The apoprotein is intrinsically disordered, which may strongly influence the clusters and the metal-thiolate (M-S) bonds, leading to a highly dynamic structure. However, these features are challenging to identify due to the transient nature of these species. The individual signal from dynamic conformations with different states of the cluster and M-S bond will be averaged and blurred in classic ensemble measurement. To circumvent these problems, we combined a single-molecule approach and multiscale molecular simulations to investigate the rupture mechanism and chemical stability of the metal cluster by a single MT molecule, focusing on the Zn4S11 cluster in the alpha domain upon unfolding. Unusual multiple unfolding pathways and intermediates are observed for both domains, corresponding to different combinations of M-S bond rupture. None of the pathways is clearly preferred suggesting that unfolding proceeds from the distribution of protein conformational substates with similar M-S bond strengths. Simulations indicate that the metal cluster may rearrange, forming and breaking metal-thiolate bonds even when MT is folded independently of large protein backbone reconfiguration. Thus, a highly dynamic polynuclear metal cluster with multiple conformational states is revealed in MT, responsible for the binding promiscuity and diverse cellular functions of this metal-carrier protein. (AU)

FAPESP's process: 17/26109-0 - Computer simulations of reactivity and electron transfer in respiratory complex II
Grantee:Felipe Curtolo
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 19/21856-7 - Computational molecular bioenergetics
Grantee:Guilherme Menegon Arantes
Support type: Regular Research Grants
FAPESP's process: 18/08311-9 - Computational bioinorganic chemistry & high-performance computing
Grantee:Guilherme Menegon Arantes
Support type: Regular Research Grants