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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Xyloglucan processing machinery in Xanthomonas pathogens and its role in the transcriptional activation of virulence factors

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Author(s):
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Vieira, Plinio S. [1] ; Bonfim, Isabela M. [2, 1] ; Araujo, Evandro A. [3, 1] ; Melo, Ricardo R. [1] ; Lima, Augusto R. [1] ; Fessel, Melissa R. [4] ; Paixao, Douglas A. A. [1] ; Persinoti, Gabriela F. [1] ; Rocco, Silvana A. [5] ; Lima, Tatiani B. [1] ; Pirolla, Renan A. S. [1] ; Morais, Mariana A. B. [6] ; Correa, Jessica B. L. [1] ; Zanphorlin, Leticia M. [1] ; Diogo, Jose A. [2, 1] ; Lima, Evandro A. [1] ; Grandis, Adriana [6] ; Buckeridge, Marcos S. [6] ; Gozzo, Fabio C. [7] ; Benedetti, Celso E. [5] ; Polikarpov, Igor [8] ; Giuseppe, Priscila O. [1] ; Murakami, Mario T. [1]
Total Authors: 23
Affiliation:
[1] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Biorenewables Natl Lab LNBR, Campinas, SP - Brazil
[2] Univ Estadual Campinas, Inst Biol, Grad Program Funct & Mol Biol, Campinas, SP - Brazil
[3] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Synchrotron Light Lab LNLS, Campinas, SP - Brazil
[4] Butantan Fdn, Butantan Inst, Sao Paulo, SP - Brazil
[5] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Biosci Natl Lab LNBio, Campinas, SP - Brazil
[6] Univ Sao Paulo, Inst Biosci, Dept Bot, Sao Paulo - Brazil
[7] Univ Estadual Campinas, Inst Chem, Campinas, SP - Brazil
[8] Univ Sao Paulo, Sao Carlos Inst Phys, Sao Carlos, SP - Brazil
Total Affiliations: 8
Document type: Journal article
Source: NATURE COMMUNICATIONS; v. 12, n. 1 JUN 30 2021.
Web of Science Citations: 0
Abstract

Xyloglucans are highly substituted and recalcitrant polysaccharides found in the primary cell walls of vascular plants, acting as a barrier against pathogens. Here, we reveal that the diverse and economically relevant Xanthomonas bacteria are endowed with a xyloglucan depolymerization machinery that is linked to pathogenesis. Using the citrus canker pathogen as a model organism, we show that this system encompasses distinctive glycoside hydrolases, a modular xyloglucan acetylesterase and specific membrane transporters, demonstrating that plant-associated bacteria employ distinct molecular strategies from commensal gut bacteria to cope with xyloglucans. Notably, the sugars released by this system elicit the expression of several key virulence factors, including the type III secretion system, a membrane-embedded apparatus to deliver effector proteins into the host cells. Together, these findings shed light on the molecular mechanisms underpinning the intricate enzymatic machinery of Xanthomonas to depolymerize xyloglucans and uncover a role for this system in signaling pathways driving pathogenesis. Xyloglucans are polysaccharides found in plant cell walls. Here, the authors describe the xyloglucan depolymerization machinery of phytopathogenic Xanthomonas bacteria, and show that sugars released by this system induce the expression of key virulence factors driving pathogenesis. (AU)

FAPESP's process: 17/14253-9 - Evaluation of the biotechnological potential of the cellulolytic system of Xanthomonas axonopodis pv. citri: a structural, functional and applied approach
Grantee:Ricardo Rodrigues de Melo
Support Opportunities: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 15/26982-0 - Exploring novel strategies for depolymerization of plant cell-wall polysaccharides: from structure, function and rational design of glycosyl hydrolases to biological implications and potential biotechnological applications
Grantee:Mário Tyago Murakami
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 16/06509-0 - Understanding the enzymatic system involved in the degradation and utilization of xyloglucans from the plant pathogen Xanthomonas axonopodis pv. citri
Grantee:Plínio Salmazo Vieira
Support Opportunities: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 15/13684-0 - Structural and functional studies of enzymes that participate in complex carbohydrates synthesis and degradation
Grantee:Igor Polikarpov
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 18/03724-3 - Understanding the molecular system of degradation and utilization of cellulose by the phytopathogen Xanthomonas citri subsp. citri
Grantee:José Alberto Diogo
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 19/13936-0 - The role of xyloglucan in the cell wall architecture determination in sugarcane
Grantee:Adriana Grandis
Support Opportunities: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 17/00203-0 - Studies of the molecular basis of mannan degradation and utilization system from the phytopathogen Xanthomonas axonopodis pv. citri
Grantee:Isabela Mendes Bonfim
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 16/19995-0 - Analysis of structural and functional diversity of GH43 enzymes from Xanthomonas axonopodis pv. citri: biological implications and potential biotechnological applications
Grantee:Mariana Abrahão Bueno de Morais
Support Opportunities: Scholarships in Brazil - Post-Doctorate