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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Responses of Low-Cost Input Combinations on the Microbial Structure of the Maize Rhizosphere for Greenhouse Gas Mitigation and Plant Biomass Production

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Yoshiura, Caio Augusto [1] ; Venturini, Andressa Monteiro [1] ; Perez Braga, Lucas Palma [1] ; da Franca, Aline Giovana [1] ; Catanho Pereira de Lyra, Maria do Carmo [2] ; Tsai, Siu Mui [1] ; Rodrigues, Jorge Luiz Mazza [3]
Total Authors: 7
[1] Univ Sao Paulo, Ctr Nucl Energy Agr, Cell & Mol Biol Lab, Piracicaba - Brazil
[2] Agron Inst Pernambuco, Genome Lab, Recife, PE - Brazil
[3] Univ Calif Davis, Soil EcoGen Lab, Davis, CA 95616 - USA
Total Affiliations: 3
Document type: Journal article
Source: FRONTIERS IN PLANT SCIENCE; v. 12, JUN 30 2021.
Web of Science Citations: 0

The microbial composition of the rhizosphere and greenhouse gas (GHG) emissions under the most common input combinations in maize (Zea mays L.) cultivated in Brazil have not been characterized yet. In this study, we evaluated the influence of maize stover coverage (S), urea-topdressing fertilization (F), and the microbial inoculant Azospirillum brasilense (I) on soil GHG emissions and rhizosphere microbial communities during maize development. We conducted a greenhouse experiment and measured methane (CH4), carbon dioxide (CO2), and nitrous oxide (N2O) fluxes from soil cultivated with maize plants under factorial combinations of the inputs and a control treatment (F, I, S, FI, FS, IS, FIS, and control). Plant biomass was evaluated, and rhizosphere soil samples were collected at V5 and V15 stages and DNA was extracted. The abundance of functional genes (mcrA, pmoA, nifH, and nosZ) was determined by quantitative PCR (qPCR) and the structure of the microbial community was assessed through 16S rRNA amplicon sequencing. Our results corroborate with previous studies which used fewer input combinations and revealed different responses for the following three inputs: F increased N2O emissions around 1 week after application; I tended to reduce CH4 and CO2 emissions, acting as a plant growth stimulator through phytohormones; S showed an increment for CO2 emissions by increasing carbon-use efficiency. IS and FIS treatments presented significant gains in biomass that could be related to Actinobacteria (19.0%) and Bacilli (10.0%) in IS, and Bacilli (9.7%) in FIS, which are the microbial taxa commonly associated with lignocellulose degradation. Comparing all factors, the IS (inoculant + maize stover) treatment was considered the best option for plant biomass production and GHG mitigation since FIS provides small gains toward the management effort of F application. (AU)

FAPESP's process: 13/22845-2 - Interactions between sugarcane soil microbiota and earthworms on straw decomposition and emissions of greenhouse gases (GHG)
Grantee:Lucas Palma Perez Braga
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 15/13546-7 - Microbial community resilience capacity assessed by metagenomics and metatranscriptomics in Amazon forest and pasture soils
Grantee:Andressa Monteiro Venturini
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 15/08564-6 - Molecular bioindicators of soil microbiome from corn crop under straw effect to N2O gas mitigation
Grantee:Caio Augusto Yoshiura
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 16/11268-2 - Uncovering microbial greenhouse gas flux mediators of the maize rhizosphere: how does Azospirillum brasiliense mitigate emissions?
Grantee:Caio Augusto Yoshiura
Support type: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 14/50320-4 - Dimensions US-BIOTA - São Paulo: collaborative research: integrating dimensions of microbial biodiversity across land use change in tropical forests
Grantee:Tsai Siu Mui
Support type: BIOTA-FAPESP Program - Thematic Grants
FAPESP's process: 19/25931-3 - Taxonomic and functional depiction of the microbial community involved in methane transformations in soils of the Brazilian Amazonia
Grantee:Andressa Monteiro Venturini
Support type: Scholarships in Brazil - Post-Doctorate