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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Impact of Three Methods of Ischemic Preconditioning on Ischemia-Reperfusion Injury in a Pig Model of Liver Transplantation

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Belon, Alessandro Rodrigo [1, 2, 3] ; Tannuri, Ana Cristina Aoun [1, 2, 3] ; de Albuquerque Rangel Moreira, Daniel [1, 2, 3] ; Figueiredo, Jose Luiz [1, 2, 3] ; da Silva, Alessandra Matheus [1, 2, 3] ; Serafini, Suellen [1, 2, 3] ; Guimaraes, Raimundo Renato [1, 2, 3] ; Faria, Caroline Silverio [1, 2, 3] ; de Alexandre, Alcione Sanches [1, 2, 3] ; Goncalves, Josiane Oliveira [1, 2, 3] ; Paes, Vitor Ribeiro [1, 2, 3] ; Tannuri, Uenis [1, 2, 3]
Total Authors: 12
[1] Univ Sao Paulo, Dept Surg, Lab Expt Surg LIM26, Sch Med, Sao Paulo - Brazil
[2] Univ Sao Paulo, Pediat Surg Div, Pediat Liver Transplantat Unit, Sch Med, Sao Paulo - Brazil
[3] Univ Sao Paulo, Lab Res Pediat Surg LIM 30, Sch Med, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of Investigative Surgery; MAY 2021.
Web of Science Citations: 0

Background Ischemic preconditioning (IPC), either direct (DIPC) or remote (RIPC), is a procedure aimed at reducing the harmful effects of ischemia-reperfusion (I/R) injury. Objectives To assess the local and systemic effects of DIPC, RIPC, and both combined, in the pig liver transplant model. Materials and methods Twenty-four pigs underwent orthotopic liver transplantation and were divided into 4 groups: control, direct donor preconditioning, indirect preconditioning at the recipient, and direct donor with indirect recipient preconditioning. The recorded parameters were: donor and recipient weight, graft-to-recipient weight ratio (GRWR), surgery time, warm and cold ischemia time, and intraoperative hemodynamic values. Blood samples were collected before native liver removal (BL) and at 0 h, 1 h, 3 h, 6 h, 12 h, 18 h, and 24 h post-reperfusion for the biochemical tests: aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), creatinine, BUN (blood urea nitrogen), lactate, total and direct bilirubin. Histopathological examination of liver, gut, kidney, and lung fragments were performed, as well as molecular analyses for expression of the apoptosis-related BAX (pro-apoptotic) and Bcl-XL (anti-apoptotic) genes, eNOS (endothelial nitric oxide synthase) gene, and IL-6 gene related to inflammatory ischemia-reperfusion injury, using real-time polymerase chain reaction (RT-PCR). Results There were no differences between the groups regarding biochemical and histopathological parameters. We found a reduced ratio between the expression of the BAX gene and Bcl-XL in the livers of animals with IPC versus the control group. Conclusions DIPC, RIPC or a combination of both, produce beneficial effects at the molecular level without biochemical or histological changes. (AU)

Grantee:Uenis Tannuri
Support type: Regular Research Grants