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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Harmful Effects of Granulocytic Myeloid-Derived Suppressor Cells on Tuberculosis Caused by Hypervirulent Mycobacteria

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Barbosa Bomfim, Caio Cesar [1] ; Amaral, Eduardo Pinheiro [2] ; Santiago-Carvalho, Igor [1] ; Santos, Gislane Almeida [1] ; Salles, Erika Machado [1] ; Hastreiter, Araceli Aparecida [3] ; do Nascimento, Rogerio Silva [1] ; Almeida, Fabricio M. [4] ; Bia Ventura Simao, Thatiana Lopes [4] ; Rezende, Andreza Linhares [4] ; Hirata, Mario Hiroyuki [3] ; Fock, Ricardo Ambrosio [3] ; Alvarez, Jose Maria [1] ; Lasunskaia, Elena B. [4] ; D'Imperio Lima, Maria Regina [1]
Total Authors: 15
[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Imunol, Sao Paulo - Brazil
[2] NIAID, Immunobiol Sect, Lab Parasit Dis, NIH, 9000 Rockville Pike, Bethesda, MD 20892 - USA
[3] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicol, Sao Paulo - Brazil
[4] Univ Estadual Norte Fluminense, Lab Biol Reconhecer, Campos Dos Goyiacazes, RJ - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Journal of Infectious Diseases; v. 223, n. 3, p. 494-507, FEB 1 2021.
Web of Science Citations: 1

Background. The role of myeloid-derived suppressor cells (MDSCs) in patients with severe tuberculosis who suffer from uncontrolled pulmonary inflammation caused by hypervirulent mycobacterial infection remains unclear. Methods. This issue was addressed using C57BL/6 mice infected with highly virulent Mycobacterium bovis strain MP287/03. Results. CD11b(+)GR1(int) population increased in the bone marrow, blood and lungs during advanced disease. Pulmonary CD11b(+)GR1(int) (Ly(6)G(int)Ly6C(int)) cells showed granularity similar to neutrophils and expressed immature myeloid cell markers. These immature neutrophils harbored intracellular bacilli and were preferentially located in the alveoli. T-cell suppression occurred concomitantly with CD11b(+)GR1(int) cell accumulation in the lungs. Furthermore, lung and bone marrow GR1(+) cells suppressed both T-cell proliferation and interferon gamma production in vitro. Anti-GR1 therapy given when MDSCs infiltrated the lungs prevented expansion and fusion of primary pulmonary lesions and the development of intragranulomatous caseous necrosis, along with increased mouse survival and partial recovery of T-cell function. Lung bacterial load was reduced by anti-GR1 treatment, but mycobacteria released from the depleted cells proliferated extracellularly in the alveoli, forming cords and clumps. Conclusions. Granulocytic MDSCs massively infiltrate the lungs during infection with hypervirulent mycobacteria, promoting bacterial growth and the development of inflammatory and necrotic lesions, and are promising targets for host-directed therapies. (AU)

FAPESP's process: 15/20432-8 - Intervention in signaling pathways associated with the recognition of cellular damage to reduce the pathology of severe forms of malaria and tuberculosis
Grantee:Maria Regina D'Império Lima
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 17/09110-4 - Morphological and Functional Characterization of Immature Myeloid Cells in Severe Tuberculosis
Grantee:Caio César Barbosa Bomfim
Support Opportunities: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 14/22986-8 - Role of myeloid derived suppressor cells and its purinergic regulation in severe tuberculosis
Grantee:Caio César Barbosa Bomfim
Support Opportunities: Scholarships in Brazil - Doctorate