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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Deciphering the role of ttrA and pduA genes for Salmonella enterica serovars in a chicken infection model

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Author(s):
Saraiva, M. M. S. [1] ; Rodrigues Alves, L. B. [1] ; Monte, D. F. M. [1] ; Ferreira, T. S. [1] ; Benevides, V. P. [1] ; Barbosa, F. O. [1] ; Freitas Neto, O. C. [2] ; Almeida, A. M. [1] ; Barrow, P. A. [3] ; Berchieri Junior, A. [1]
Total Authors: 10
Affiliation:
[1] Sao Paulo State Univ FCAV Unesp, Lab Avian Pathol, Dept Pathol Theriogenol & One Hlth, Jaboticabal - Brazil
[2] Fed Univ Minas Gerais UFMG, Vet Sch, Dept Prevent Vet Med, Belo Horizonte, MG - Brazil
[3] Univ Surrey, Sch Vet Med & Sci, Guildford, Surrey - England
Total Affiliations: 3
Document type: Journal article
Source: AVIAN PATHOLOGY; v. 50, n. 3, p. 257-268, MAY 4 2021.
Web of Science Citations: 0
Abstract

Salmonella enterica serovars use self-induced intestinal inflammation to increase electron acceptor availability and to obtain a growth advantage in the host gut. There is evidence suggesting that the ability of Salmonella to use tetrathionate and 1,2-propanediol provides an advantage in murine infection. Thus, we present here the first study to evaluate both systemic infection and faecal excretion in commercial poultry challenged by Salmonella Enteritidis (SE) and S. Typhimurium (STM) harbouring deletions in ttrA and pduA genes, which are crucial to the metabolism of tetrathionate and 1,2-propanediol, respectively. Mutant strains were excreted at higher rates when compared to the wild-type strains. The highest rates were observed with white egg-layer and brown egg-layer chicks (67.5%), and broiler chicks (56.7%) challenged by SE Delta ttrA Delta pduA, and brown egg-layer chicks (64.8%) challenged by STM Delta ttrA Delta pduA. SE Delta ttrA Delta pduA presented higher bacterial counts in the liver and spleen of the three chicken lineages and caecal contents from the broiler chickens, whereas STM Delta ttrA Delta pduA presented higher counts in the liver and spleen of the broiler and brown-egg chickens for 28 days post-infection (P < 0.05). The ttrA and pduA genes do not appear to be major virulence determinants in faecal excretion or invasiveness for SE and STM in chickens. (AU)

FAPESP's process: 20/06076-2 - Evaluation of avian infection (Gallus Gallus domesticus) by Salmonella enteritidis, Salmonella typhimurium and Salmonella Heidelberg containing deletion of the ttrA and pduA genes
Grantee:Daniel Farias Marinho Do Monte
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 18/21301-2 - Evaluation of avian infection (Gallus gallus domesticus) by Salmonella enteritidis, Salmonella typhimurium and Salmonella heidelberg containing deletion of the ttrA and pduA genet
Grantee:Mauro de Mesquita Souza Saraiva
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 18/03189-0 - Evaluation of avian infection (Gallus gallus domesticus) by Salmonella enteritidis, Salmonella typhimurium and Salmonella heidelberg containing deletion of the ttrA and pduA genes
Grantee:Angelo Berchieri Junior
Support type: Research Projects - Thematic Grants