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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

erobic growth physiology of Saccharomyces cerevisiae on sucrose is strain-dependen

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Soares Rodrigues, Carla Ines [1, 2] ; Wahl, Aljoscha [2] ; Gombert, Andreas K. [1]
Total Authors: 3
[1] Univ Estadual Campinas, Sch Food Engn, Rua Monteiro Lobato 80, BR-13083862 Campinas, SP - Brazil
[2] Delft Univ Technol, Dept Biotechnol, Van der Maasweg 9, NL-2629 HZ Delft - Netherlands
Total Affiliations: 2
Document type: Journal article
Source: FEMS Yeast Research; v. 21, n. 3 MAY 2021.
Web of Science Citations: 1

Present knowledge on the quantitative aerobic physiology of the yeast Saccharomyces cerevisiae during growth on sucrose as sole carbon and energy source is limited to either adapted cells or to the model laboratory strain CEN.PK113-7D. To broaden our understanding of this matter and open novel opportunities for sucrose-based biotechnological processes, we characterized three strains, with distinct backgrounds, during aerobic batch bioreactor cultivations. Our results reveal that sucrose metabolism in S. cerevisiae is a strain-specific trait. Each strain displayed distinct extracellular hexose concentrations and invertase activity profiles. Especially, the inferior maximum specific growth rate (0.21 h(-1)) of the CEN.PK113-7D strain, with respect to that of strains UFMG-CM-Y259 (0.37 h(-1)) and JP1 (0.32 h(-1)), could be associated to its low invertase activity (0.04-0.09 U/mg(DM)). Moreover, comparative experiments with glucose or fructose alone, or in combination, suggest mixed mechanisms of sucrose utilization by the industrial strain JP1, and points out the remarkable ability of the wild isolate UFMG-CM-259 to grow faster on sucrose than on glucose in a well-controlled cultivation system. This work hints to a series of metabolic traits that can be exploited to increase sucrose catabolic rates and bioprocess efficiency. (AU)

FAPESP's process: 17/18206-5 - Turbo yeast: fast glycolytic rates displayed by sucrose-grown Saccharomyces cerevisiae
Grantee:Carla Ines Soares Rodrigues
Support Opportunities: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 17/08464-7 - Sucrose metabolism in Saccharomyces cerevisiae: towards synthetic yeasts with faster glycolytic rates for sucrose-based industrial biotechnology
Grantee:Andreas Karoly Gombert
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
FAPESP's process: 16/07285-9 - Why does not Saccharomyces cerevisiae grow faster on glucose than on sucrose?
Grantee:Carla Ines Soares Rodrigues
Support Opportunities: Scholarships in Brazil - Doctorate