Advanced search
Start date
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish

Full text
Fenero, Camila Morales [1] ; Padovani, Barbara Nunes [1] ; do Amaral, Mariana Abrantes [1, 2] ; Bottura de Barros, Guilherme Jose [1] ; Xavier de Oliveira, Izabella Karina [1] ; Hiyane, Meire Ioshie [1] ; Saraiva Camara, Niels Olsen [1, 2]
Total Authors: 7
[1] Univ Sao Paulo, Dept Immunol, Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, Dept Med, Nephrol Div, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Web of Science Citations: 0

Cisplatin is commonly used as chemotherapy. Although it has positive effects in cancer-treated individuals, cisplatin can easily accumulate in the kidney due to its low molecular weight. Such accumulation causes the death of tubular cells and can induce the development of Acute Kidney Injury (AKI), which is characterized by a quick decrease in kidney function, tissue damage, and immune cells infiltration. If administered in specific doses cisplatin can be a useful tool as an AKI inducer in animal models. The zebrafish has appeared as an interesting model to study renal function, kidney regeneration, and injury, as renal structures conserve functional similarities with mammals. Adult zebrafish injected with cisplatin shows decreased survival, kidney cell death, and increased inflammation markers after 24 h post-injection (hpi). In this model, immune cells infiltration and cell death can be assessed by flow cytometry and TUNEL assay. This protocol describes the procedures to induce AKI in adult zebrafish by intraperitoneal cisplatin injection and subsequently demonstrates how to collect the renal tissue for flow cytometry processing and cell death TUNEL assay. These techniques will be useful to understand the effects of cisplatin as a nephrotoxic agent and will contribute to the expansion of AKI models in adult zebrafish. This model can also be used to study kidney regeneration, in the search for compounds that treat or prevent kidney damage and to study inflammation in AKI. Moreover, the methods used in this protocol will improve the characterization of tissue damage and inflammation, which are therapeutic targets in kidney-associated comorbidities. (AU)

FAPESP's process: 17/05687-5 - Influence of microbiota on the role of macrophages during acute renal injury induced by cisplatin in zebrafish
Grantee:Guilherme José Bottura de Barros
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 15/21644-9 - Obesity-induced inflammation, alterations in the microbiota and their effects on the enteric nervous system: study in an experimental model of zebrafish.
Grantee:Camila Idelí Morales Fénero
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 18/20722-4 - Study of the effects of diet rich in fructose and vitamin's role in regulating inflammation in acute renal injury model in zebrafish
Grantee:Mariana Abrantes do Amaral
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 17/05264-7 - Cell metabolism, microbiota and immune system: new paradigms in renal diseases physiopathology
Grantee:Niels Olsen Saraiva Câmara
Support Opportunities: Research Projects - Thematic Grants