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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Osmotic and ionic regulation, and kinetic characteristics of a posterior gill (Na+, K+)-ATPase from the blue crab Callinectes danae on acclimation to salinity challenge

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Author(s):
Garcon, Daniela P. [1] ; Leone, Francisco A. [2] ; Faleiros, Rogerio O. [3] ; Pinto, Marcelo R. [4] ; Moraes, Cintya M. [5] ; Fabri, Leonardo M. [5] ; Antunes, Claudia D. [6] ; McNamara, John Campbell [7, 6]
Total Authors: 8
Affiliation:
[1] Univ Fed Triangulo Mineiro, Campus Univ Iturama, Iturama, MG - Brazil
[2] Univ Sao Paulo, Dept Quim, Fac Filosofia Ciencias & Letras Ribeirao Preto, Ribeirao Preto, SP - Brazil
[3] Univ Fed Espirito Santo, Dept Ciencias Agr & Biol, Sao Mateus, ES - Brazil
[4] Univ Uberaba, Lab Biopatol & Biol Mol, Uberaba, MG - Brazil
[5] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Bioquim & Imunol, Ribeirao Preto, SP - Brazil
[6] Univ Sao Paulo, Dept Biol, Fac Filosofia Ciencias & Letras Ribeirao Preto, Ave Bandeirantes 3900, BR-14040901 Ribeirao Preto, SP - Brazil
[7] Univ Sao Paulo, Ctr Biol Marinha, Sao Sebastiao, SP - Brazil
Total Affiliations: 7
Document type: Journal article
Source: Marine Biology; v. 168, n. 6 JUN 2021.
Web of Science Citations: 0
Abstract

Salt tolerance reflects ecophysiological adaptation, and the wide-ranging distribution of the Brachyura mirrors their ability to adjust body fluid concentrations. The gill (Na+, K+)-ATPase underpins such hyper/hypo-regulatory mechanisms. We evaluate osmotic and chloride regulation in Callinectes danae after 10 days acclimation to a wide salinity range (5-50 parts per thousand S), accompanying alterations in hemolymph osmolality and {[}Cl-] during hypo- (15 parts per thousand S) or hyper- (45 parts per thousand S) osmotic challenge. Further, we investigate posterior gill (Na+, K+)-ATPase kinetics, alpha-subunit immunolocalization and its mRNA and protein expression (15, 30 and 40 or 45 parts per thousand S). The crab is a moderate, asymmetrical hyper/hypo-osmoregulator but is a strong, asymmetrical hyper/hypo-chloride regulator. Hyper-regulation at low salinity is sustained by a threefold increase in (Na+, K+)-ATPase activity, a 3.5-fold increase in alpha-subunit mRNA expression and 1.6-fold increase in protein expression. alpha-Subunit signal is highest in 15 parts per thousand S-acclimated crabs, and is uniformly distributed throughout the ionocytes and pillar cells. Activity in 30- and 40% S-acclimated crabs is similar. Affinity for ATP and Na+ increases on high salinity acclimation but decreases for ouabain. K+ apparent affinity is independent of salinity, while that for Mg2+ decreases and for NH4+ increases with increasing salinity. A high-affinity ATP-binding site disappears on acclimation at any salinity. FOF1- and Na+- or K+-ATPase activities decrease with increased salinity. Hemolymph chloride hypo-regulation depends little on gill (Na+, K+)-ATPase activity. Hyper-, and hypo-osmotic and ionic regulatory capabilities in C. danae are intricate physiological processes underpinned by multifarious gill (Na+, K+)-ATPase kinetics and altered mRNA and protein expressions. (AU)

FAPESP's process: 15/00131-3 - Chloride secretion by the gill epithelium of shrimps and crabs from different osmotic niches: a physiological and molecular investigation of the Na+-K+-2Cl- symporter
Grantee:John Campbell McNamara
Support type: Regular Research Grants
FAPESP's process: 07/04870-9 - Adaptive physiological strategies in the Crustacea: the invasion of fresh water and the molecular and structural bases of osmoregulation
Grantee:John Campbell McNamara
Support type: Regular Research Grants
FAPESP's process: 16/25336-0 - Comparative biochemical study of gill (Na+, K +)- ATPase of Macrobrachium amazonicum (Heller, 1862) inhabitant of coastal and continental regions
Grantee:Francisco de Assis Leone
Support type: Regular Research Grants