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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

PAR gamma-induced upregulation of subcutaneous fat adiponectin secretion, glyceroneogenesis and BCAA oxidation requires mTORC1 activit

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Author(s):
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Andrade, Maynara L. [1] ; Gilio, Gustavo R. [1] ; Perandini, Luiz A. [1] ; Peixoto, Albert S. [1] ; Moreno, Mayara F. [1] ; Castro, Erique [1] ; Oliveira, Tiago E. [1] ; Vieira, Thayna S. [1] ; Ortiz-Silva, Milene [1] ; Thomazelli, Caroline A. [1] ; Chaves-Filho, Adriano B. [2, 1] ; Belchior, Thiago [1] ; Chimin, Patricia [3] ; Magdalon, Juliana [4] ; Ivison, Rachael [5] ; Pant, Deepti [2] ; Tsai, Linus [2] ; Yoshinaga, Marcos Y. [6] ; Miyamoto, Sayuri [6] ; Festuccia, William T. [1]
Total Authors: 20
Affiliation:
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, Av Prof Lineu Prestes 1524, BR-05508000 Sao Paulo - Brazil
[2] Beth Israel Deaconess Med Ctr, Dept Med, Div Endocrinol Diabet & Metab, Boston, MA 02215 - USA
[3] Univ Estadual Londrina, Phys Educ & Sports Ctr, Dept Phys Educ, Londrina, Parana - Brazil
[4] Hosp Israelita Albert Einstein, Sao Paulo, SP - Brazil
[5] Broad Inst MIT & Harvard, Cambridge, MA 02142 - USA
[6] Univ Sao Paulo, Inst Chem, Dept Biochem, Sao Paulo - Brazil
Total Affiliations: 6
Document type: Journal article
Source: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS; v. 1866, n. 8 AUG 2021.
Web of Science Citations: 2
Abstract

The nutrient sensors peroxisome proliferator-activated receptor. (PPAR gamma) and mechanistic target of rapamycin complex 1 (mTORC1) closely interact in the regulation of adipocyte lipid storage. The precise mechanisms underlying this interaction and whether this extends to other metabolic processes and the endocrine function of adipocytes are still unknown. We investigated herein the involvement of mTORC1 as a mediator of the actions of the PPAR gamma ligand rosiglitazone in subcutaneous inguinal white adipose tissue (iWAT) mass, endocrine function, lipidome, transcriptome and branched-chain amino acid (BCAA) metabolism. Mice bearing regulatory associated protein of mTOR (Raptor) deletion and therefore mTORC1 deficiency exclusively in adipocytes and littermate controls were fed a high-fat diet supplemented or not with the PPAR gamma agonist rosiglitazone (30 mg/kg/day) for 8 weeks and evaluated for iWAT mass, lipidome, transcriptome (Rnaseq), respiration and BCAA metabolism. Adipocyte mTORC1 deficiency not only impaired iWAT adiponectin transcription, synthesis and secretion, PEPCK mRNA levels, triacylglycerol synthesis and BCAA oxidation and mRNA levels of related proteins but also completely blocked the upregulation in these processes induced by pharmacological PPAR gamma activation with rosiglitazone. Mechanistically, adipocyte mTORC1 deficiency impairs PPAR gamma transcriptional activity by reducing PPAR gamma protein content, as well as by downregulating C/EBP alpha, a co-partner and facilitator of PPAR gamma. In conclusion, mTORC1 and PPAR gamma are essential partners involved in the regulation of subcutaneous adipose tissue adiponectin production and secretion and BCAA oxidative metabolism. (AU)

FAPESP's process: 17/17582-3 - Characterization of the involvement of nutrient sensor mTORC1 in the hepatic steatosis, steatohepatitis, and hepatocarcinoma induced by PTEN deletion into hepatocytes
Grantee:Mayara Franzoi Moreno
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 16/23169-9 - Involvement of mTORC1 and 2 and PPARgamma in the regulation of leukocyte profile, recruitment and activation of brown adipose tissue and beige adipocytes induced by cold exposure
Grantee:Érique de Castro
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 15/13508-8 - Involvement of adipocytes mTOR complexes 1 and 2 in the morphological, metabolic and secretory changes induced by pharmacological PPARgamma activation
Grantee:Maynara Lucca Andrade
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 19/17660-0 - Discovery of new transcription factors involved in the development of steatosis, steatopatite and hepatocarcinome
Grantee:Luiz Augusto Buoro Perandini
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 17/17403-1 - Characterization of the molecular mechanisms by which TSC1 deletion in adipocytes increases oxidative metabolism and UCP1 content in white adipose tissue
Grantee:Thayna dos Santos Vieira
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 13/07937-8 - Redoxome - Redox Processes in Biomedicine
Grantee:Ohara Augusto
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 19/04271-5 - UCP1-independent mechanisms involved in the increased energy expenditure and protection against Obesity induced by fish oil in mice
Grantee:Tiago Eugênio Oliveira da Silva
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 18/11156-5 - Effects of obesity and severe lipodystrophy on oxidation and peroxidation of lipids: involvement of these molecules on inflammation and insulin resistance
Grantee:Adriano de Britto Chaves Filho
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 15/19530-5 - Involvement of the nutrient sensor mTOR in the development of obesity associated chronic metabolic diseases
Grantee:William Tadeu Lara Festuccia
Support type: Research Projects - Thematic Grants
FAPESP's process: 19/01763-4 - Characterization of the molecular mechanisms determining the energy balance, glucose homeostasis and hepatomegaly in mice with severe lipodystrophy
Grantee:William Tadeu Lara Festuccia
Support type: Regular Research Grants
FAPESP's process: 15/22983-1 - Discovery of new transcription factors involved in the development of steatosis, steatohepatitis, and liver cancer
Grantee:Thiago Belchior de Oliveira
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 17/12260-8 - Autophagy involvement in the metabolic and inflammatory alterations in adipose tissue associated with Obesity induced by high fat diet
Grantee:Milene Ortiz Silva
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 17/23040-9 - Characterization of mTORC2 role in the hepatic steatosis, steatohepatitis and hepatocellular carcinoma progression induced by PTEN deletion in hepatocytes
Grantee:Álbert Souza Peixoto
Support type: Scholarships in Brazil - Doctorate