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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Field Deployment of a Mobile Biosafety Laboratory Reveals the Co-Circulation of Dengue Viruses Serotype 1 and Serotype 2 in Louga City, Senegal, 2017

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Author(s):
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Dieng, Idrissa [1] ; Diarra, Maryam [2] ; Diagne, Moussa Moise [1] ; Faye, Martin [1] ; Dior Ndione, Marie Henriette [1] ; Ba, Yamar [3] ; Diop, Mamadou [2] ; Ndiaye, El Hadji [3] ; Marinho de Andrade Zanotto, Paolo [4] ; Diop, Boly [5] ; Ndiaye, Mamadou [5] ; Bousso, Abdoulaye [5] ; Dia, Ndongo [1] ; Diallo, Mawlouth [3] ; Barry, Aliou [4] ; Fall, Gamou [1] ; Loucoubar, Cheikh [2] ; Sall, Amadou Alpha [1] ; Faye, Ousmane [1] ; Faye, Oumar [1]
Total Authors: 20
Affiliation:
[1] Inst Pasteur, Dept Virol, Arboviruses & Haemorrhag Fever Viruses Unit, Dakar 220 - Senegal
[2] Inst Pasteur, Epidemiol Clin Res & Data Sci Unit, Dakar 220 - Senegal
[3] Inst Pasteur, Med Entomol Unit, 36 Ave, Dakar - Senegal
[4] Univ SP, Inst Biomed Sci, Dept Microbiol, Lab Mol Evolut & Bioinformat, Sao Paulo, SP - Brazil
[5] Minist Hlth, Prevent Dept, Dakar - Senegal
Total Affiliations: 5
Document type: Journal article
Source: JOURNAL OF TROPICAL MEDICINE; v. 2021, APR 1 2021.
Web of Science Citations: 2
Abstract

Dengue virus (DENV) is the most prevalent arboviral threat worldwide. This virus belonging to genus Flavivirus, Flaviviridae family, is responsible for a wide spectrum of clinical manifestations, ranging from asymptomatic or mild febrile illness (dengue fever) to life-threatening infections (severe dengue). Many sporadic cases and outbreaks have occurred in Senegal since 1970. Nevertheless, this article describes a field investigation of suspected dengue cases, between 05 September 2017 and 17 December 2017 made possible by the deployment of a Mobile Biosafety Laboratory (MBS-Lab). Overall, 960 human sera were collected and tested in the field for the presence of viral RNA by real-time RT-PCR. Serotyping, sequencing of complete E gene, and phylogenetic analysis were also performed. Out of 960 suspected cases, 131 were confirmed dengue cases. The majority of confirmed cases were from Louga community. Serotyping revealed two serotypes, Dengue 1 (100/104; 96, 15%) and Dengue 2 (04/104; 3, 84%). Phylogenetic analysis of the sequences obtained indicated that the Dengue 1 strain was closely related to strains isolated, respectively, in Singapore (Asia) in 2013 (KX380803.1) outbreak and it cocirculated with a Dengue 2 strain closely related to strains from a Burkina Faso dengue outbreak in 2016 (KY62776.1). Our results showed the co-circulation of two dengue virus serotypes during a single outbreak in a short time period. This co-circulation highlighted the need to improve surveillance in order to prevent future potential severe dengue cases through antibody-dependent enhancement (ADE). Interestingly, it also proved the reliability and usefulness of the MBS-Lab for expedient outbreak response at the point of need, which allows early cases management. (AU)

FAPESP's process: 17/23281-6 - The use of antibodies that neutralize infection at a post-attachment step for the development of innovative immunotherapeutic strategies exemplified for selective destruction of Zika-infected human cells.
Grantee:Paolo Marinho de Andrade Zanotto
Support Opportunities: Regular Research Grants