Silva, Thayna Souza
Moura, Claudia L.
Ambrosio, Sergio Ricardo
Sola Veneziani, Rodrigo Cassio
de Paiva, Raphael E. F.
Bastos, Jairo Kenupp
Gomes Martins, Carlos Henrique
Total Authors: 8
 Univ Franca, Res Lab Appl Microbiol, Franca, SP - Brazil
 Univ Franca, Nucleus Res Sci & Technol, Franca, SP - Brazil
 Univ Sao Paulo, Inst Chem, Dept Fundamental Chem, Sao Carlos, SP - Brazil
 Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Ribeirao Preto, SP - Brazil
 Univ Fed Uberlandia, Inst Biomed Sci ICBIM, Lab Antimicrobial Testing, Av Para 1720, BR-38405320 Uberlandia, MG - Brazil
Total Affiliations: 5
MAR 2 2021.
Web of Science Citations:
Invasion of periodontal tissues by Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans can be associated with aggressive forms of periodontitis. Oleoresins from different copaifera species and their compounds display various pharmacological properties. The present study evaluates the antibacterial and antivirulence activity of oleoresins obtained from different copaifera species and of ten isolated compounds against two causative agents of periodontitis. The following assays were performed: determination of the minimum inhibitory concentration (MIC), determination of the minimum bactericidal concentration (MBC), and determination of the antibiofilm activity by inhibition of biofilm formation and biofilm eradication tests. The antivirulence activity was assessed by hemagglutination, P. gingivalis Arg-X and Lis-X cysteine protease inhibition assay, and A. actinomycetemcomitans leukotoxin inhibition assay. The MIC and MBC of the oleoresins and isolated compounds 1, 2, and 3 ranged from 1.59 to 50 mu g/mL against P. gingivalis (ATCC 33277) and clinical isolates and from 6.25 to 400 mu g/mL against A. actinomycetemcomitans (ATCC 43717) and clinical isolates. About the antibiofilm activity, the oleoresins and isolated compounds 1, 2, and 3 inhibited biofilm formation by at least 50% and eradicated pre-formed P. gingivalis and A. actinomycetemcomitans biofilms in the monospecies and multispecies modes. A promising activity concerning cysteine protease and leucotoxin inhibition was also evident. In addition, molecular docking analysis was performed. The investigated oleoresins and their compounds may play an important role in the search for novel sources of agents that can act against periodontal pathogens. (AU)