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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Cystic proliferation of germline stem cells is necessary to reproductive success and normal mating behavior in medaka

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Author(s):
Padilla, Luisa F. Arias [1] ; Castaneda-Cortes, Diana C. [2] ; Rosa, Ivana F. [2] ; Acosta, Omar D. Moreno [3] ; Hattori, Ricardo S. [2] ; Nobrega, Rafael H. [1] ; Fernandino, I, Juan
Total Authors: 7
Affiliation:
[1] I, INTECH CONICET UNSAM, Inst Tecnol Chascomus, Chascomus - Argentina
[2] Sao Paulo State Univ UNESP, Inst Biosci, Dept Struct & Funct Biol, Reprod & Mol Biol Grp, Botucatu, SP - Brazil
[3] Sao Paulo Fisheries Inst APTA SAA, UPD CJ, Salmonid Expt Stn Campos do Jordao, Campos Do Jordao - Brazil
Total Affiliations: 3
Document type: Journal article
Source: eLIFE; v. 10, MAR 1 2021.
Web of Science Citations: 0
Abstract

The production of an adequate number of gametes is necessary for normal reproduction, for which the regulation of proliferation from early gonadal development to adulthood is key in both sexes. Cystic proliferation of germline stem cells is an especially important step prior to the beginning of meiosis; however, the molecular regulators of this proliferation remain elusive in vertebrates. Here, we report that ndrg1b is an important regulator of cystic proliferation in medaka. We generated mutants of ndrg1b that led to a disruption of cystic proliferation of germ cells. This loss of cystic proliferation was observed from embryogenic to adult stages, impacting the success of gamete production and reproductive parameters such as spawning and fertilization. Interestingly, the depletion of cystic proliferation also impacted male sexual behavior, with a decrease of mating vigor. These data illustrate why it is also necessary to consider gamete production capacity in order to analyze reproductive behavior. (AU)

FAPESP's process: 18/10265-5 - Endocrine and molecular mechanisms involved in the sexual differentiation of Medaka (Oryzias latipes) under conditions of thermal stress
Grantee:Ivana Felipe da Rosa
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 14/07620-7 - Spermatogonial stem cell bank to preserve the genotype of endangered and valuable teleost species
Grantee:Rafael Henrique Nóbrega
Support Opportunities: Research Grants - Young Investigators Grants