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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Dengue-2 and Guadeloupe Mosquito Virus RNA Detected in Aedes (Stegomyia) spp. Collected in a Vehicle Impound Yard in Santo Andre, SP, Brazil

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Author(s):
Rangel, Marina E. O. [1] ; Oliveira, Luana P. R. [2] ; Cabral, Aline D. [2] ; Gois, Katharyna C. [3] ; Lima, Marcos V. M. [1] ; Reis, Beatriz C. A. A. [3] ; Fonseca, Fernando L. A. [3, 4] ; Speranca, Marcia A. [2] ; Gehrke, Flavia S. [5, 6, 7] ; Laporta, Gabriel Z. [1]
Total Authors: 10
Affiliation:
[1] Ctr Univ Saude ABC FMABC, Fundacao ABC, Setor Posgrad Pesquisa & Inovacao, BR-09060870 Santo Andre, SP - Brazil
[2] Univ Fed ABC UFABC, Ctr Ciencias Nat & Humanas, BR-09606045 Sao Bernardo Do Campo - Brazil
[3] Ctr Univ Saude ABC FMABC, Lab Analises Clin, Fundacao ABC, BR-09060870 Santo Andre, SP - Brazil
[4] Univ Fed Sao Paulo UNIFESP, Dept Farm, BR-09972270 Diadema - Brazil
[5] Ctr Univ Saude ABC FMABC, Dept Patol, Fundacao ABC, BR-09060870 Santo Andre, SP - Brazil
[6] Hosp Servidor Publ Estadual IAMSPE, Programa Posgrad Ciencias Saude, BR-04039000 Sao Paulo - Brazil
[7] Univ Paulista UNIP, Dept Farm, BR-01504001 Sao Paulo - Brazil
Total Affiliations: 7
Document type: Journal article
Source: INSECTS; v. 12, n. 3 MAR 2021.
Web of Science Citations: 0
Abstract

Simple Summary We screened Aedes mosquitoes in an urban area in southeastern Brazil for emerging arboviruses using RT-PCR assays and sequencing. We detected DENV-2 RNA in Aedes albopictus and Guadeloupe mosquito virus RNA in Aedes aegypti. In 2018-2019, we conducted mosquito collections in a municipal vehicle impound yard, which is 10 km from the Serra do Mar Environmental Protection Area in Santo Andre, SP, Brazil. Our aim is to study arboviruses in the impound yard, to understand the transmission of arboviruses in an urban environment in Brazil. We captured the mosquitoes using human-landing catches and processed them for arbovirus detection by conventional and quantitative RT-PCR assays. We captured two mosquito species, Aedes aegypti (73 total specimens; 18 females and 55 males) and Ae. albopictus (34 specimens; 27 females and 7 males). The minimum infection rate for DENV-2 was 11.5 per 1000 (CI95%: 1-33.9). The detection of DENV-2 RNA in an Ae. albopictus female suggests that this virus might occur in high infection rates in the sampled mosquito population and is endemic in the urban areas of Santo Andre. In addition, Guadeloupe mosquito virus RNA was detected in an Ae. aegypti female. To our knowledge, this was the first detection of the Guadeloupe mosquito virus in Brazil. (AU)

FAPESP's process: 16/14514-4 - Characterization of the chitinase from South American endemic Leishmania species: use in diagnosis in humans, dogs and sandflies.
Grantee:Marcia Aparecida Speranca
Support type: Regular Research Grants
FAPESP's process: 19/11384-0 - Investigation of alphaviruses in hematophagous insects
Grantee:Luana Prado Rolim de Oliveira
Support type: Scholarships in Brazil - Master
FAPESP's process: 16/14457-0 - Development of a diagnostic platform and monitoring system for arboviruses in the city of Santo André, Brazil
Grantee:Flávia de Sousa Gehrke
Support type: Regular Research Grants