Inst Butantan, Lab Herpetol, Ave Vital Brazil 1500, BR-05503900 Sao Paulo, SP - Brazil
 Univ Sao Paulo, Inst Butantan, Inst Pesquisas Tecnol, Interunidades Biotecnol, Sao Paulo - Brazil
 Univ Fed Sao Paulo, Dept Bioquim, Sao Paulo - Brazil
Total Affiliations: 3
APR 15 2021.
Web of Science Citations:
The venom color variation of Crotalus durissus terrificus (Cdt) is attributed to the presence of the toxin L-amino acid oxidase (LAAO). During the venom milking routine of Institute Butantan, we have noticed that most venoms of captive Cdt specimens show a yellowish color, while most venoms of wild specimens are white. Here we describe a comparative analysis of long-term captive (LTC) and recently wild-caught (RWC) Cdt, focusing on LAAO variation. For the identification of LAAO in individual venoms, four different approaches were employed: evaluation of the enzymatic activity, SDS-PAGE, Western blotting, and ELISA. In addition, mass spectrometry analysis was performed using pooled samples. Although some variation among these methodologies was observed, it was possible to notice that the presence of LAAO was significantly higher in the venom of LTC individuals. LAAO was identified in 60-80% LTC specimens and in only 10-12% of RWC specimens. Furthermore, this enzyme accounts for 5.6% of total venom proteins of LTC Cdt pooled venom, while it corresponds to only 0.7% of RWC Cdt pooled venom. These findings strongly suggest that captive maintenance increases the expression of LAAO in Cdt venom. (AU)