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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Chalcone T4, a novel chalconic compound, inhibits inflammatory bone resorption in vivo and suppresses osteoclastogenesis in vitro

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Fernandes, Natalie Aparecida Rodrigues [1] ; Camilli, Angelo Constantino [1] ; Maldonado, Laura Andrea Gonzalez [1] ; Pacheco, Cindy Grace Perez [1] ; Silva, Amanda Favoreto [1] ; Molon, Rafael Scaf [1] ; Spolidorio, Luiz Carlos [2] ; Ribeiro de Assis, Leticia [3] ; Regasini, Luis Octavio [3] ; Rossa Junior, Carlos [1] ; Guimaraes-Stabili, Morgana Rodrigues [1]
Total Authors: 11
[1] Sao Paulo State Univ UNESP, Sch Dent Araraquara, Dept Diag & Surg, Rua Humaita, 1680 Ctr, BR-14801903 Araraquara, SP - Brazil
[2] Sao Paulo State Univ UNESP, Sch Dent Araraquara, Dept Physiol & Pathol, Araraquara, SP - Brazil
[3] Sao Paulo State Univ UNESP, Dept Chem & Environm Sci, Sao Jose Do Rio Preto, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Web of Science Citations: 0

Objective: This study aimed to assess the effect of a novel synthetic chalcone, Chalcone T4, on a murine model of periodontitis and on RANKL-induced osteoclastogenesis in vitro. Background: Chalcones are natural compounds with anti-inflammatory properties, and its synthetic analogs with enhanced biological effects have potential as therapeutic agents. Periodontitis is characterized by chronic inflammation of the periodontium and alveolar bone resorption. Safe and effective anti-inflammatory agents can have an important additive effect in the treatment in this disease. Methods: Periodontitis was induced via the installation of a ligature around the first molar. Rats (n = 32) received Chalcone T4 (5 and 50 mg/kg) or distilled water by gavage daily for 15 days. Outcomes assessed were bone resorption (mu CT), TNF-alpha production (ELISA), cellular infiltrate, and collagen content (stereometric analysis, CD45+ cells by immunohistochemistry), and activation of NFATc1 and NF-kB (immunohistochemistry). In vitro, RAW 264.7 were treated with Chalcone T4 and stimulated with RANKL for assessment of osteoclast differentiation (actin ring staining) and activity (pit assay). Results: Chalcone T4 significantly reduced periodontitis-associated bone resorption, as well as the cellular infiltrate, while increasing the collagen content. Production of TNF-alpha, infiltration of CD45-positive cells, and NF-kB activation were markedly reduced. In vitro, chalcone T4 inhibited both osteoclast differentiation and activity. Conclusion: Chalcone T4 significantly inhibited alveolar bone resorption and inflammation in vivo and RANKL-induced osteoclastogenesis in vitro, suggesting a therapeutic role for this compound in the treatment of periodontitis. (AU)

FAPESP's process: 18/17047-3 - Effect of a novel Chalcone on inflammatory bone resorption in an experimental model of periodontal disease.
Grantee:Morgana Rodrigues Guimarães Stabili
Support Opportunities: Regular Research Grants