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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Paracoccidioides brasiliensis Releases a DNase-Like Protein That Degrades NETs and Allows for Fungal Escape

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Zonta, Yohan Ricci [1] ; Dezen, Ana Laura Ortega [1] ; Della Coletta, Amanda Manoel [1] ; Yu, Kaio Shu Tsyr [1] ; Carvalho, Larissa [1] ; Santos, Leandro Alves dos [2] ; Depra, Igor de Carvalho [3] ; Kratofil, Rachel M. [4, 5, 6] ; Willson, Michelle Elizabeth [4, 5, 6] ; Zbytnuik, Lori [4, 5, 6] ; Kubes, Paul [4, 5, 6] ; Ximenes, Valdecir Farias [7] ; Dias-Melicio, Luciane Alarcao [1, 2, 8]
Total Authors: 13
[1] Sao Paulo State Univ UNESP, Lab Immunopathol & Infect Agents LIAI, UNIPEX Expt Res Unity, Med Sch Botucatu, Sect 5, Botucatu, SP - Brazil
[2] Sao Paulo State Univ UNESP, Confocal Microscopy Lab, UNIPEX Expt Res Unity, Med Sch Botucatu, Botucatu, SP - Brazil
[3] Sao Paulo State Univ UNESP, Lab Genet Basis Endocrinol Dis, Expt Res Unity UNIPEX, Sect 5, Botucatu, SP - Brazil
[4] Univ Calgary, Calvin Phoebe & Joan Snyder Inst Chron Dis, Calgary, AB - Canada
[5] Univ Calgary, Cumming Sch Med, Dept Physiol & Pharmacol, Calgary, AB - Canada
[6] Univ Calgary, Dept Microbiol Immunol & Infect Dis, Cumming Sch Med, Calgary, AB - Canada
[7] Sao Paulo State Univ UNESP, Dept Chem, Sch Sci, Bauru, SP - Brazil
[8] Sao Paulo State Univ UNESP, Dept Pathol, Med Sch Botucatu, Botucatu, SP - Brazil
Total Affiliations: 8
Document type: Journal article
Web of Science Citations: 0

Paracoccidioidomycosis is a systemic fungal disease, considered endemic in Latin America. Its etiological agents, fungi of the Paracoccidioides complex, have restricted geographic habitat, conidia as infecting form, and thermo-dimorphic characteristics. Polymorphonuclear neutrophils (PMNs) are responsible for an important defense response against fungus, releasing Neutrophil Extracellular Traps (NETs), which can wrap and destroy the yeasts. However, it has been described that some pathogens are able to evade from these DNA structures by releasing DNase as an escape mechanism. As different NETs patterns have been identified in PMNs cultures challenged with different isolates of Paracoccidioides brasiliensis, the general objective of this study was to identify if different patterns of NETs released by human PMNs challenged with Pb18 (virulent) and Pb265 (avirulent) isolates would be correlated with fungal ability to produce a DNase-like protein. To this end, PMNs from healthy subjects were isolated and challenged in vitro with both fungal isolates. The production, release, and conformation of NETs in response to the fungi were evaluated by Confocal Microscopy, Scanning Microscopy, and NETs Quantification. The identification of fungal DNase production was assessed by DNase TEST Agar, and the relative gene expression for hypothetical proteins was investigated by RT-qPCR, whose genes had been identified in the fungal genome in the GenBank (PADG\_11161 and PADG\_08285). It was possible to verify the NETs release by PMNs, showing different NETs formation when in contact with different isolates of the fungus. The Pb18 isolate induced the release of looser, larger, and more looking like degraded NETs compared to the Pb265 isolate, which induced the release of denser and more compact NETs. DNase TEST Agar identified the production of a DNase-like protein, showing that only Pb18 showed the capacity to degrade DNA in these plates. Besides that, we were able to identify that both PADG\_08528 and PADG\_11161 genes were more expressed during interaction with neutrophil by the virulent isolate, being PADG\_08528 highly expressed in these cultures, demonstrating that this gene could have a greater contribution to the production of the protein. Thus, we identified that the virulent isolate is inducing more scattered and loose NETs, probably by releasing a DNase-like protein. This factor could be an important escape mechanism used by the fungus to escape the NETs action. (AU)

FAPESP's process: 18/09706-7 - Identification and purification of DNAse produced by fungi of the Paracoccidioides spp complex and their action on NETs (Neutrophil Extracellular Traps) released by human neutrophils in response to the fungus
Grantee:Luciane Alarcão Dias-Melicio
Support Opportunities: Regular Research Grants
FAPESP's process: 17/26230-3 - Action of DNase produced by Paracoccidioides Brasiliensis on nets (neutrophil extracellular traps) released by human neutrophils in response to the fungus
Grantee:Yohan Ricci Zonta
Support Opportunities: Scholarships in Brazil - Master