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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Promising potential of articaine-loaded poly(epsilon-caprolactone) nanocapules for intraoral topical anesthesia

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da Silva, Camila Batista [1, 2] ; Volpato, Maria Cristina [1] ; Muniz, Bruno Vilela [1, 3] ; dos Santos, Cleiton Pita [1] ; Serpe, Luciano [4, 1] ; Nunes Ferreira, Luiz Eduardo [1, 5] ; Silva de Melo, Nathalie Ferreira [6, 7] ; Fraceto, Leonardo Fernandes [6] ; Groppo, Francisco Carlos [1] ; Franz-Montan, Michelle [1]
Total Authors: 10
[1] Univ Estadual Campinas, UNICAMP, Piracicaba Dent Sch, Dept Biosci, Piracicaba, SP - Brazil
[2] Univ Mogi das Cruzes UMC, Hlth Sci, Mogi Das Cruzes, SP - Brazil
[3] Itapeva Fac Social & Agr Sci FAIT, Itapeva, SP - Brazil
[4] Univ Estadual Ponta Grossa, Dept Dent, Ponta Grossa, Parana - Brazil
[5] Guarulhos Univ UNG, Lab Inflammat & Immunol, Guarulhos, SP - Brazil
[6] Sao Paulo State Univ, Dept Environm Engn, Sorocaba, SP - Brazil
[7] Sao Leopoldo Mandic Res Inst, Dept Immunol & Mol Biol, Campinas, SP - Brazil
Total Affiliations: 7
Document type: Journal article
Source: PLoS One; v. 16, n. 2 FEB 11 2021.
Web of Science Citations: 0

To determine whether the permeation capacity and analgesic efficacy of articaine (ATC) could be increased and cytotoxicity decreased by encapsulation in poly(e-caprolactone) nanocapsules (ATC(nano)), aiming at local or topical anesthesia in dentistry. Cellular viability was evaluated (using the MTT test and fluorescence microscopy) after 1 h and 24 h exposure of HaCaT cells to ATC, ATC(nano), ATC with epinephrine (ATC(epi)), and ATC in nanocapsules with epinephrine (ATC(nanoepi)). The profiles of permeation of 2% ATC and 2% ATC(nano) across swine esophageal epithelium were determined using Franz-type vertical diffusion cells. Analgesic efficacy was evaluated with a von Frey anesthesiometer in a postoperative pain model in rats, comparing the 2% ATC, 2% ATC(nano), 2% ATC(epi), and 2% ATC(nanoepi) formulations to 4% ATC(epi) (a commercially available formulation). We show that use of the nanocapsules decreased the toxicity of articaine (P<0.0001) and increased its flux (P = 0.0007). The 2% ATC(epi) and 4% ATC(epi) formulations provided higher analgesia success and duration (P<0.05), compared to 2% ATC, 2% ATC(nano), and 2% ATC(nanoepi). Articaine-loaded poly(e-caprolactone) nanocapsules constitute a promising formulation for intraoral topical anesthesia (prior to local anesthetic injection), although it is not effective when injected in inflamed tissues for pain control, such as irreversible pulpitis. (AU)

FAPESP's process: 12/06974-4 - Development, biocompatibility and permeation studies on gel formulations of poly-epsilon-caprolactone nanocapsules containing local anesthetics
Grantee:Michelle Franz Montan Braga Leite
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 12/02539-1 - Biocompatibity and permeation studies of articaine-loaded poly(epsilon-caprolactone) nanocapules formulations.
Grantee:Camila Batista da Silva de Araujo Candido
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 12/07310-2 - Modulatory effects of local anesthetics associated with carriers on the production of inflammatory mediators and cell viability in oral epithelial cells and human gingival fibroblasts
Grantee:Francisco Carlos Groppo
Support Opportunities: Regular Research Grants