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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Influence of the capping of biogenic silver nanoparticles on their toxicity and mechanism of action towards Sclerotinia scierotiorum

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Author(s):
Guilger-Casagrande, Mariana [1, 2] ; Germano-Costa, Tais [1] ; Bilesky-Jose, Natalia [1] ; Pasquoto-Stigliani, Tatiane [1] ; Carvalho, Lucas [2] ; Fraceto, Leonardo F. [2] ; de Lima, Renata [1]
Total Authors: 7
Affiliation:
[1] Univ Sorocaba, Lab Evaluat Bioact & Toxicol Nanomat, Sorocaba, SP - Brazil
[2] Sao Paulo State Univ, Lab Environm Nanotechnol, Sorocaba, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: JOURNAL OF NANOBIOTECHNOLOGY; v. 19, n. 1 FEB 24 2021.
Web of Science Citations: 0
Abstract

Background: Biogenic nanoparticles possess a capping of biomolecules derived from the organism employed in the synthesis, which contributes to their stability and biological activity. These nanoparticles have been highlighted for the control of phytopathogens, so there is a need to understand their composition, mechanisms of action, and toxicity. This study aimed to investigate the importance of the capping and compare the effects of capped and uncapped biogenic silver nanoparticles synthesized using the filtrate of Trichoderma harzianum against the phytopathogenic fungus Sclerotinia sclerotiorum. Capping removal, investigation of the composition of the capping and physico-chernical characterization of the capped and uncapped nanoparticles were performed. The effects of the nanoparticles on S. scierotiorum were evaluated in vitro. Cytotoxicity and genotoxicity of the nanoparticles on different cell lines and its effects on nontarget microorganisms were also investigated. Results: The capped and uncapped nanoparticles showed spherical morphology, with greater diameter of the uncapped ones. Functional groups of biomolecules, protein bands and the hydrolytic enzymes NAGase, beta-1,3-glucanase, chitinase and acid protease from T. harzianum were detected in the capping. The capped nanoparticles showed great inhibitory potential against S. scierotiorum, while the uncapped nanoparticles were ineffective. There was no difference in cytotoxicity comparing capped and uncapped nanoparticles, however higher genotoxicity of the uncapped nanoparticles was observed towards the cell lines. Regarding the effects on nontarget microorganisms, in the minimal inhibitory concentration assay only the capped nanoparticles inhibited microorganisms of agricultural importance, while in the molecular analysis of the soil microbiota there were major changes in the soils exposed to the uncapped nanoparticles. Conclusions: The results suggest that the capping played an important role in controlling nanoparticle size and contributed to the biological activity of the nanoparticles against S. scierotiorum. This study opens perspectives for investigations concerning the application of these nanoparticles for the control of phytopathogens. (AU)

FAPESP's process: 16/13692-6 - Silver biogenic nanoparticles based on Trichoderma harzianum: different synthesis conditions, evaluation of cyto/genotoxicity and inhibitory potential of Sclerotinia sclerotiorum
Grantee:Mariana Guilger Casagrande
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 18/23608-8 - Development of labelled nanopesticides aiming studies of mechanism of action and toxicity
Grantee:Lucas Bragança de Carvalho
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 17/20932-6 - Green synthesis and evaluation of iron nanoparticles based on Trichoderma harzianum for application in agriculture
Grantee:Natalia Bilesky José
Support type: Scholarships in Brazil - Master
FAPESP's process: 17/13328-5 - Development and evaluation of biogenic metal nanoparticles (silver, titanium and iron) based on Trichoderma harzianum for agricultural application
Grantee:Renata de Lima
Support type: Regular Research Grants
FAPESP's process: 20/05816-2 - Study of the capping of biogenic nanoparticles and their correlation with toxicity and activity in the control of phytopathogens
Grantee:Mariana Guilger Casagrande
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 17/21004-5 - Agriculture, micro/nanotechnology and environment: from evaluation of the mechanisms of action to studies of transport and toxicity
Grantee:Leonardo Fernandes Fraceto
Support type: Research Projects - Thematic Grants