| Full text | |
| Author(s): |
Leastro, Mikhail Oliveira
[1, 2]
;
Freitas-Astua, Juliana
[1, 3]
;
Kitajima, Elliot Watanabe
[4]
;
Pallas, Vicente
[2]
;
Sanchez-Navarro, Jesus A.
[2]
Total Authors: 5
|
| Affiliation: | [1] Inst Biol, Unidade Lab Referencia Biol Mol Aplicada, BR-04014002 Sao Paulo, SP - Brazil
[2] Univ Politecn Valencia, CSIC, Inst Biol Mol & Celular Plantas, Valencia 46022 - Spain
[3] Lab Fitopatol Embrapa Mandioca & Frutic, BR-44380000 Cruz Das Almas, BA - Brazil
[4] Univ Sao Paulo, Escola Super Agr Luiz Queiroz, Dept Fitopatol & Nematol, BR-13418900 Piracicaba, SP - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | MICROORGANISMS; v. 9, n. 2 FEB 2021. |
| Web of Science Citations: | 1 |
| Abstract | |
Although citrus leprosis disease has been known for more than a hundred years, one of its causal agents, citrus leprosis virus C2 (CiLV-C2), is poorly characterized. This study described the association of CiLV-C2 movement protein (MP) and capsid protein (p29) with biological membranes. Our findings obtained by computer predictions, chemical treatments after membrane fractionation, and biomolecular fluorescence complementation assays revealed that p29 is peripherally associated, while the MP is integrally bound to the cell membranes. Topological analyses revealed that both the p29 and MP expose their N- and C-termini to the cell cytoplasmic compartment. The implications of these results in the intracellular movement of the virus were discussed. (AU) | |
| FAPESP's process: | 15/10249-1 - Studies of infective processes of Citrus leprosis virus (CiLV-C), with emphasis in the construction of infectious clone and characterization of viral proteins. |
| Grantee: | Mikhail Oliveira Leastro |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 17/19898-8 - The involvement of the movement protein (MP) of Citrus leprosis-associated viruses with the natural restraint of systemic viral spread in the plant. |
| Grantee: | Mikhail Oliveira Leastro |
| Support Opportunities: | Scholarships abroad - Research Internship - Post-doctor |
| FAPESP's process: | 17/50222-0 - Understanding the molecular biology and ecology of plant-virus-vector relationships: towards sustainable, integrated virus management strategies |
| Grantee: | Juliana de Freitas Astúa |
| Support Opportunities: | Regular Research Grants |