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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Profiling plasma-extracellular vesicle proteins and microRNAs in diabetes onset in middle-aged male participants in the ELSA-Brasil study

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Masi, Laureane N. [1] ; Lotufo, Paulo A. [2] ; Ferreira, Frederico M. [3] ; Rodrigues, Alice C. [4] ; Serdan, Tamires D. A. [1] ; Souza-Siqueira, Talita [1] ; Braga, Aecio A. [5] ; Saldarriaga, Magda E. G. [5] ; Alba-Loureiro, Tatiana C. [1] ; Borges, Fernanda T. [1] ; Cury, Diego P. [6] ; Hirata, Mario H. [5] ; Gorjao, Renata [1] ; Pithon-Curi, Tania C. [1] ; Lottenberg, Simao A. [7] ; Fedeli, Ligia M. G. [2] ; Nakaya, I, Helder T. ; Bensenor, Isabela J. M. [2] ; Curi, Rui [8, 1] ; Hirabara, Sandro M. [1]
Total Authors: 20
Affiliation:
[1] Cruzeiro Univ, Interdisciplinary Postgrad Program Hlth Sci, Sao Paulo - Brazil
[2] Univ Sao Paulo, Ctr Clin & Epidemiol Res, Sao Paulo - Brazil
[3] Univ Sao Paulo, Sch Med, Dept Pathol, Sao Paulo - Brazil
[4] I, Univ Sao Paulo, Dept Pharmacol, Inst Biomed Sci, Sao Paulo, SP - Brazil
[5] Univ Sao Paulo, Fac Pharmaceut Sci, Sao Paulo - Brazil
[6] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, Sao Paulo - Brazil
[7] Univ Sao Paulo, Hosp Clin, Fac Med, Sao Paulo - Brazil
[8] Butantan Inst, Sao Paulo - Brazil
Total Affiliations: 8
Document type: Journal article
Source: PHYSIOLOGICAL REPORTS; v. 9, n. 3 FEB 2021.
Web of Science Citations: 0
Abstract

We measured plasma-derived extracellular vesicle (EV) proteins and their microRNA (miRNA) cargos in normoglycemic (NG), glucose intolerant (GI), and newly diagnosed diabetes mellitus (DM) in middle-aged male participants of the Brazilian Longitudinal Study of Adult Health (ELSA-Brazil). Mass spectrometry revealed decreased IGHG-1 and increased ITIH2 protein levels in the GI group compared with that in the NG group and higher serotransferrin in EVs in the DM group than in those in the NG and GI groups. The GI group also showed increased serum ferritin levels, as evaluated by biochemical analysis, compared with those in both groups. Seventeen miRNAs were differentially expressed (DEMiRs) in the plasma EVs of the three groups. DM patients showed upregulation of miR-141-3p and downregulation of miR-324-5p and -376c-3p compared with the NG and GI groups. The DM and GI groups showed increased miR-26b-5p expression compared with that in the NG group. The DM group showed decreased miR-374b-5p levels compared with those in the GI group and higher concentrations than those in the NG group. Thus, three EV proteins and five DEMiR cargos have potential prognostic importance for diabetic complications mainly associated with the immune function and iron status of GI and DM patients. (AU)

FAPESP's process: 14/21447-6 - microRNAs identification as potential targets of type 2 diabetes mellitus diagnostic and therapeutic
Grantee:Laureane Nunes Masi
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 19/25892-8 - Reversal of spontaneous Type 2 Diabetes by bone marrow transplant
Grantee:Laureane Nunes Masi
Support type: Regular Research Grants
FAPESP's process: 15/12728-4 - Characterization of epigenetics biomarkers of type 2 diabetes mellitus: identification of potential therapeutic targets of n-3 polyunsaturated fatty acids
Grantee:Sandro Massao Hirabara
Support type: Regular Research Grants
FAPESP's process: 17/03707-9 - Metabolic memory for atherosclerosis is mediated by epigenetic modifications in the bone marrow
Grantee:Laureane Nunes Masi
Support type: Scholarships abroad - Research Internship - Post-doctor
FAPESP's process: 18/00998-5 - Characterization of epigenetic biomarkers of type 2 diabetes mellitus: identification of potential therapeutic targets of n-3 polyunsaturated fatty acids
Grantee:Tatiana Carolina Alba Loureiro
Support type: Scholarships in Brazil - Technical Training Program - Technical Training