Advanced search
Start date
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Influence of high glucose in the expression of miRNAs and IGF1R signaling pathway in human myometrial explants

Full text
Favaro, Rodolfo R. [1, 2] ; Morales-Prieto, Diana M. [2] ; Herrmann, Joerg [3] ; Sonnemann, Juergen [4] ; Schleussner, Ekkehard [2] ; Markert, Udo R. [2] ; Zorn, Telma M. T. [1]
Total Authors: 7
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Cell & Dev Biol, Lab Reprod & Extracellular Matrix Biol, Sao Paulo - Brazil
[2] Jena Univ Hosp, Dept Obstet, Placenta Lab, Jena - Germany
[3] Hufeland Klinikum, Dept Gynecol & Obstet, Weimar - Germany
[4] Jena Univ Hosp, Dept Pediat Hematol & Oncol, Childrens Clin, Jena - Germany
Total Affiliations: 4
Document type: Journal article
Web of Science Citations: 0

Purpose Several roles are attributed to the myometrium including sperm and embryo transport, menstrual discharge, control of uterine blood flow, and labor. Although being a target of diabetes complications, the influence of high glucose on this compartment has been poorly investigated. Both miRNAs and IGF1R are associated with diabetic complications in different tissues. Herein, we examined the effects of high glucose on the expression of miRNAs and IGF1R signaling pathway in the human myometrium. Methods Human myometrial explants were cultivated for 48 h under either high or low glucose conditions. Thereafter, the conditioned medium was collected for biochemical analyses and the myometrial samples were processed for histological examination as well as miRNA and mRNA expression profiling by qPCR. Results Myometrial structure and morphology were well preserved after 48 h of cultivation in both high and low glucose conditions. Levels of lactate, creatinine, LDH and estrogen in the supernatant were similar between groups. An explorative screening by qPCR arrays revealed that 6 out of 754 investigated miRNAs were differentially expressed in the high glucose group. Data validation by single qPCR assays confirmed diminished expression of miR-215-5p and miR-296-5p, and also revealed reduced miR-497-3p levels. Accordingly, mRNA levels of IGF1R and its downstream mediators FOXO3 and PDCD4, which are potentially targeted by miR-497-3p, were elevated under high glucose conditions. In contrast, mRNA expression of IGF1, PTEN, and GLUT1 was unchanged. Conclusions The human myometrium responds to short-term exposure (48 h) to high glucose concentrations by regulating the expression of miRNAs, IGF1R and its downstream targets. (AU)

FAPESP's process: 14/23517-1 - Does type 1 diabetes change the microRNA expression profile on the uterus of ovariectomized mice submitted or not to estrogen replacement?
Grantee:Rodolfo Favaro Ribeiro
Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
FAPESP's process: 13/16922-4 - Immunohistochemical characterization of extracellular matrix components during development of deciduoma and study of the effect of ovarian hormones on the expression and synthesis of extracellular matrix molecules by decidual cells in vitro
Grantee:Telma Maria Tenório Zorn
Support Opportunities: Regular Research Grants