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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Identification of novel mRNA isoforms associated with meat tenderness using RNA sequencing data in beef cattle

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Author(s):
Magalhaes Muniz, Maria Malane [1, 2] ; Simielli Fonseca, Larissa Fernanda [1] ; dos Santos Silva, Danielly Beraldo [1] ; de Oliveira, Hinayah Rojas [2] ; Baldi, Fernando [3, 1] ; Chardulo, Artur Loyola [3, 4] ; Ferro, Jesus Aparecido [3, 1] ; Canovas, Angela [2] ; de Albuquerque, Lucia Galvao [3, 1]
Total Authors: 9
Affiliation:
[1] Sao Paulo State Univ Unesp, Sch Agr & Vet Sci, Jaboticabal, SP - Brazil
[2] Univ Guelph, Ctr Genet Improvement Livestock, Dept Anim Biosci, Guelph, ON - Canada
[3] Natl Council Sci & Technol Dev CNPq, Brasilia, DF - Brazil
[4] Sao Paulo State Univ Unesp, Coll Vet & Anim Sci, Botucatu, SP - Brazil
Total Affiliations: 4
Document type: Review article
Source: MEAT SCIENCE; v. 173, MAR 2021.
Web of Science Citations: 0
Abstract

The Warner-Bratzler shear force (WBSF) and myofibrillar fragmentation index (MFI) are complementary methodologies used to measure beef tenderness. Longissimus thoracis samples from the 20 most extreme bulls (out of 80 bulls set) for WBSF (tender (n = 10) and tough (n = 10)) and MFI (high (n = 10) and low (n = 10)) traits were collected to perform transcriptomic analysis using RNA-Sequencing. All analysis were performed through CLC Genomics Workbench. A total of 39 and 27 transcripts for WBSF and MFI phenotypes were DE, respectively. The possible DE novel mRNA isoforms, for WBSF and MFI traits, are myosin encoders (e.g. MYL1 and MYL6). In addition, we identified potential mRNA isoforms related to genes affecting the speed fibers degradation during the meat aging process. The DE novel transcripts are transcripted by genes with biological functions related to oxidative process, energy production and striated muscle contraction. The results suggest that the identified mRNA isoforms could be used as potential candidate to select animals in order to improve meat tenderness. (AU)

FAPESP's process: 18/20026-8 - Multi-user equipment approved in grant 2017/10630-2: server
Grantee:Lucia Galvão de Albuquerque
Support type: Multi-user Equipment Program
FAPESP's process: 11/21241-0 - Study of the genetic variability of meat fatty acid profile in Nelore cattle finished in feedlot
Grantee:Fernando Sebastián Baldi Rey
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 18/11154-2 - Identification of co-expression genes in Nellore cattle muscle tissue with divergent marbling and myofibrilar fragmentation index phenotypes, using RNA-Seq data
Grantee:Maria Malane Magalhães Muniz
Support type: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 09/16118-5 - Genomic tools to genetic improvement of direct economic important traits in Nelore cattle
Grantee:Lucia Galvão de Albuquerque
Support type: Research Projects - Thematic Grants
FAPESP's process: 17/10630-2 - Genetic aspects of meat production quality, efficiency and sustainability in Nelore breed animals
Grantee:Lucia Galvão de Albuquerque
Support type: Research Projects - Thematic Grants