| Author(s): |
de Freitas Guaitolini, Carlos Renato
[1]
;
Dias Maziero, Rosiara Rosaria
[1]
;
de Souza Castilho, Anthony Cesar
[2]
;
Pagnano Derussi, Ana Augusta
[3]
;
Volpato, Rodrigo
[4]
;
Ackermann, Camila Louise
[4]
;
Matsubara, Lidia Mitsuko
[4]
;
Lopes, Maria Denise
[4]
Total Authors: 8
|
| Affiliation: | [1] Univ Paranaense, Umuarama, PR - Brazil
[2] Oeste Paulista Univ, Presidente Prudente, SP - Brazil
[3] Jose do Rosario Vellano Univ, Alfenas, MG - Brazil
[4] FMVZ UNESP, Dept Radiol & Anim Reprod, Botucatu, SP - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | CRYOLETTERS; v. 41, n. 6, p. 330-336, NOV 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
BACKGROUND: Canine embryo cryopreservation and subsequent transfer are relevant in the use of reproductive technologies. OBJECTIVE: The purpose of this study is the identification and quantification of the gene expression BAX and Bcl2, AQP3, Na+/K+ ATPase alpha 1 and beta 1 and LIFr in canine embryos obtained in vivo and after freezing. MATERIALS AND METHODS: For the collection of embryos, the bitches were identified at pro-estrous until the detection of 80-90% superficial cells. After that, they were artificially inseminated with fresh semen. The embryos were collected after ovariohysterectomy. RNA was extracted and amplified, and embryos were randomly distributed into fresh (Fr) and frozen/thawed (Ft) groups. RESULTS: Eighteen blastocysts were collected from three bitches. Genes BAX, AQP3 and LIFr did not differ among the studied groups. CONCLUSION: We suggest, through these results, that the genes BAX, Bcl2, AQP3, Na + / K + ATPase alpha 1 and beta 1 and LIFr were expressed in canine blastocysts collected in vivo and after slow freezing cryopreservation. (AU) | |
| FAPESP's process: | 12/03912-8 - Effects of slow freezing and vitrification in canine embryos |
| Grantee: | Maria Denise Lopes |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 11/08798-6 - Effects of slow freezing and vitrification on viability, ultrastructure and gene expression of canine embryos |
| Grantee: | Carlos Renato de Freitas Guaitolini |
| Support Opportunities: | Scholarships in Brazil - Doctorate |