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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Peripheral Blood miRome Identified miR-155 as Potential Biomarker of MetS and Cardiometabolic Risk in Obese Patients

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Cerda, Alvaro [1, 2, 3] ; Amaral, Adonai Aralim [3] ; de Oliveira, Raquel [3] ; Moraes, Tamiris Invencioni [3] ; Braga, Aecio Assuncao [3] ; Graciano-Saldarriaga, Magda Elizabeth [3] ; Fajardo, Cristina Moreno [3] ; Hirata, Thiago Dominguez Crespo [3] ; Bonezi, Vivian [3] ; Campos-Salazar, Antony Brayan [3] ; Dorea, Egidio Lima [4] ; Bernik, Marcia Martins Silveira [4] ; Hirata, Mario Hiroyuki [3] ; Hirata, Rosario Dominguez Crespo [3]
Total Authors: 14
[1] Univ La Frontera, CEMT BIOREN, Ctr Excellence Translat Med, Av Alemania 0458, Temuco 4810296 - Chile
[2] Univ La Frontera, Dept Basic Sci, Av Alemania 0458, Temuco 4810296 - Chile
[3] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, Av Prof Lineu Prestes 580, BR-05508000 Sao Paulo - Brazil
[4] Univ Sao Paulo, Univ Hosp, Av Prof Lineu Prestes 2565, BR-05508000 Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Web of Science Citations: 0

This study explored circulating miRNAs and target genes associated with metabolic syndrome (MetS) and cardiometabolic risk in obese patients. Small-RNA sequencing was used to assess the peripheral blood miRNome of 12 obese subjects (6 MetS and 6 non-MetS). Differentially expressed miRNAs and target genes were further analyzed by qPCR in a larger sample of obese patients (48 MetS and 32 non-MetS). miRNA:mRNA interactions were studied using in silico tools. miRNome analysis identified 10 downregulated miRNAs in MetS compared to non-Met patients (p < 0.05). In silico studies revealed three miRNAs (miR-155, miR-181a, and let-7a) and their predictive targets (CCAAT/enhancer-binding protein beta-CEBPB, KRAS proto-oncogene, GTPase-KRAS and suppressor of cytokine signaling 1-SOCS1) with a potential role in the insulin receptor signaling pathway. miR-155 expression was reduced and CEBPB mRNA levels were increased in MetS patients (p < 0.05), and these effects were correlated with the number of MetS diagnostic criteria (p < 0.05). Increased HOMA-IR (>7.6) was associated with low miR-155 levels, high CEBPB expression, and serum hsCRP (p < 0.05). miR-155 was negatively correlated with CEBPB, HOMA-IR, and plasma fibrinogen, and positively correlated with serum adiponectin (p < 0.05). Downregulation of circulating miR-155 is associated with insulin resistance, poor glycemic control, and increased MetS-related cardiometabolic risk, and these effects are potentially mediated by interaction with CEBPB. (AU)

FAPESP's process: 09/10069-2 - Influence of genetic makers on response to a dietary intervention to reduce body weight
Grantee:Rosario Dominguez Crespo Hirata
Support Opportunities: Regular Research Grants