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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

ntergenerational thyroid hormone homeostasis imbalance in cerebellum of rats perinatally exposed to glyphosate-based herbicid

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Author(s):
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Costa Reis, Lua Taina [1] ; Sena de Souza, Janaina [2] ; Hirochi Herai, Roberto [3, 4] ; Cunha, Eduardo Brunetti [3] ; Ribeiro Pereira Soares, Janaina [5] ; Santos El-Bacha, Ramon [5] ; Diogenes Amaral da Silva, Victor [5] ; Aurelio Romano, Marco [6] ; Marino Romano, Renata [6] ; Izabel Chiamolera, Maria [2] ; Giannocco, Gisele [2] ; Lima Costa, Silvia [5] ; Dias da Silva, Magnus Regios [2] ; Telles da Cunha Lima, Suzana [1, 7]
Total Authors: 14
Affiliation:
[1] Univ Fed Bahia UFBA, Inst Biol, Lab Bioprospeccao & Biotecnol, Salvador, BA - Brazil
[2] Univ Fed Sao Paulo UNIFESP EPM, Disciplina Endocrinol Clin, Dept Med, Sao Paulo - Brazil
[3] Pontificia Univ Catolica Parana PUCPR, Sch Med, Grad Program Hlth Sci, Curitiba, Parana - Brazil
[4] Lico Kaesemodel Inst, Res Dept, Curitiba, Parana - Brazil
[5] Univ Fed Bahia UFBA, Lab Neuroquim & Biol Celular, Salvador, BA - Brazil
[6] Univ Estadual Ctr Oeste UNICENTRO, Dept Farm, Guarapuava - Brazil
[7] AnaclinGENE, Curitiba, Parana - Brazil
Total Affiliations: 7
Document type: Journal article
Source: ENVIRONMENTAL TOXICOLOGY; v. 36, n. 6 JAN 2021.
Web of Science Citations: 1
Abstract

Agrochemicals became a public health concern due to increased human exposure and possible endocrine disruption effects in several organs, including the brain. Thyroid hormones controls neurodevelopment, which turn them sensitive to endocrine disruptors (EDs). In this work, we evaluated the effect of glyphosate-based herbicides (GBH) as an intergenerational endocrine disrupter on thyroid homeostasis in cerebellar cells. Female pregnant Wistar rats were exposed to Roundup Transorb (R) solution at 5 and 50 mg/kg/day, from gestation day 18 to post-natal day 5 (P5). Cerebellum of male offspring was used to evaluate gene expression. The mRNA levels of thyroid hormone receptors, hormonal conversion enzymes, hormone transporters, as well as, de novo epigenetic regulators were altered, with some of these genes presenting a non-monotonic dose response. Furthermore, metabolomic profile correlation with tested dose demonstrated altered metabolic profile, in agreement with cerebellar gene alterations. Moreover, cerebellar primary cultures exposed to non-toxic GBH concentration presented a decrease level in glial fibrillary acidic protein, a protein regulated by endocrine signals. In conclusion, our results indicate that animals exposed to non-toxic GBH doses during perinatal phase carry intergenerational alterations in key regulators of cellular thyroid hormone homeostasis and epigenetic controllers in adulthood, indicating the possible ED effect of GBH based on epigenetic alterations. (AU)

FAPESP's process: 13/26851-7 - In vivo study of the effects of the endocrine disruptors glyphosate and bisphenol-A on the hypothalamic-pituitary-thyroid axis
Grantee:Maria Izabel Chiamolera
Support type: Regular Research Grants
FAPESP's process: 17/07053-3 - Effect of thyroid hormone on the brain of 3xTg-AD mice, model of Alzheimer's Disease, on glucose and cholesterol metabolism
Grantee:Janaína Sena de Souza
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 18/22763-0 - Ancient DNA, bioengineering and brain "organoids": study of thyroid hormone homeostasis and Alzheimer's Disease markers in NOVA1 gene CRISPR edited stem cells
Grantee:Janaína Sena de Souza
Support type: Scholarships abroad - Research Internship - Post-doctor