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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Ruthenium red attenuates brown adipose tissue thermogenesis in rats

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Lillo Vizin, Robson Cristiano [1] ; Carrettiero, Daniel Carneiro [1, 2] ; Ishikawa, Debora T. [1] ; Cruz-Neto, Ariovaldo P. [3] ; Almeida, Maria Camila [1, 2]
Total Authors: 5
[1] Univ Fed ABC, Grad Program Neurosci & Cognit, Sao Bernardo Do Campo, SP - Brazil
[2] Univ Fed ABC, Nat & Humanities Sci Ctr, Rua Arcturus 03, BR-09606 Sao Bernardo Do Campo, SP - Brazil
[3] Sao Paulo State Univ, Biosci Inst, Dept Zool, Rio Claro, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of Thermal Biology; v. 95, JAN 2021.
Web of Science Citations: 0

Ruthenium red (RR) is a non-selective antagonist of the temperature-sensitive Transient Receptor Potential (TRP) channels and it is an important pharmacological tool in thermoregulatory research. However, the effect of RR on thermoeffector activity is not well established. Here we evaluated the effect of RR on cold-defense thermoeffectors induced by menthol, an agonist of the cold-sensitive TRPM8 channel. Adult male Wistar rats were used. Epidermal treatment with menthol raised deep body temperature due to an increase in oxygen consumption (an index of thermogenesis), a reduction in heat loss index (an index of cutaneous vasoconstriction), and an induction in warmth-seeking behavior in a two-temperature choice apparatus. Pretreatment with RR attenuated the menthol-induced increase in deep body temperature and oxygen consumption, but it did not affect heat loss index and warmth-seeking behavior. To stimulate brown adipose tissue thermogenesis, rats were treated with CL 316,243, a potent and selective beta 3-adrenoceptor agonist. CL 316,243 increased deep body temperature, which was attenuated by RR pretreatment. We conclude that RR reduces brown adipose tissue thermogenesis induced by menthol and CL 316,243, independent of effects at the thermal sensor level (i.e., TRPM8). (AU)

FAPESP's process: 15/23426-9 - Beta-amyloid in Alzheimer's Disease: death or survival? Involvement of NF-kappaB and BAG2
Grantee:Daniel Carneiro Carrettiero
Support type: Regular Research Grants
FAPESP's process: 13/25503-5 - Involvement of TRPM8 channels in thermoregulation of Wistar rats
Grantee:Robson Cristiano Lillo Vizin
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 15/02991-0 - Involvement of TRPM8 channels in thermoregulation of Wistar rats
Grantee:Maria Camila Almeida
Support type: Regular Research Grants